穗花蛇菰的抗發炎成分研究   全文下載

Anti-Inflammatory Principles from Balanophora laxiflora

由穗花蛇菰（蛇菰科植物）分離鑑定18個已知化合物，其中包括有木脂素，苯丙素，三?類和甾醇等化合物。進一步對這些化學成分進行一氧化氮（NO）形成的抑制活性評價。異落葉松樹脂醇(1)，乙基咖啡酸(13)和阿魏醛(16)對NO的產生，分別具有輕度到強度的抑制作用，其IC50值分別為0.81，7.29和98.67 μM；化合物1顯示了最強的抑制NO產生作用，同時，對TNF-α產生亦有很強的抑制作用，其IC50值為0.87 μM，但是化合物1對IL-6並沒有顯著影響。此外，經由β-lactamase reporter活性分析發現化合物1可顯著抑制 NF-κB的活性。綜合以上，結果表明化合物1可能是透過衰減NF-κB的轉錄作用，而調節促炎介質的產生。

Eighteen known compounds, including lignans, phenylpropanoids, triterpenoids, and phytosterols, were isolated from Balanophora laxiflora (Balanophoraceae). The inhibitory activities of these compounds on the production of nitric oxide (NO), a pro-inflammatory mediator, were evaluated. Compounds isolariciresinol (1), ethyl caffeate (13) and ferulic aldehyde (16) showed potent to mild inhibitory activities on LPS-stimulated NO production in RAW 264.7 macrophages with IC50 values of 0.81, 7.29, and 98.67 μM, respectively. The results showed that compound 1 had a potent effect on TNF-α production with an IC50 value of 0.87 μM, but practically no effect on IL-6 synthesis. In addition, an inhibitory effect of compound 1 on nuclear factor-κB (NF-κB) activation was shown by the repression of LPS-stimulated β-lactamase reporter activity. Taken together, these results demonstrated that compound 1 modulates the production of inflammatory mediators through the attenuation of NF-κB transcription signaling.