微生物鑑定系統檢測牛奶中抗生素殘留   全文下載

Microbial System for Identification of Antibiotic Residues in Milk

本研究目的在評鑑ResScreen(R) 微生物鑑定系統應用於牛奶中抗生素殘留檢驗。該微生物系統由BT（betalactams and tetracyclines）及BS（betalac-tams and sulfamides）兩種生物測定方法組成，包含G. stearothermophilus subsp. Calidolactis孢子，培養基和指示劑（酸鹼及氧化還原）等，以邏輯迴歸分析模式計算29種抗菌劑之偵測極限。兩 種 方 法 均 可 檢 測penicillin-G, ampicillin, amoxicillin, cloxacillin, oxacillin, cephalexin, cefoperazone及ceftiofur(R)等之殘留量，且趨近其最大殘留極限（MRL），BT生物測定法對tetracycline及oxytetracycline殘留呈現良好敏感性，而BS生物測定法可檢測牛奶中sulfadiazine, sulfamethoxazole及sulfathiazole殘留。同時使用這兩種生物測定法可檢測牛奶中betalactam, tetracycline及sulfamide殘留；neomycin, tylosin及lincomycin的殘留亦可被偵測到，由於微生物的敏感性，這些分子在BT和BS兩種生物測定法中均如betalac-tams，呈陽性反應。"

The aim of this study was to evaluate the ResScreen(R) microbiological system for the identification of antibiotic residues in milk. This microbiological system consists of two methods, the BT (betalactams and tetracyclines) and BS (betalactams and sulfamides) bioassays, containing spores of G. stearothermophilus subsp. calidolactis, culture media and indicators (acid-base and redox). The detection limits of 29 antimicrobial agents were calculated using a logistic regression model. Both methods detect residues of penicillin-G, ampicillin, amoxicillin, cloxacillin, oxacillin, cephalexin, cefoperazone and ceft-iofur(R) at levels close to their Maximum Residue Limits (MRL). The BT bioassay also presents good sensitivity to tetracycline and oxytetracycline residues, whereas the BS bioassay detects sulfadiazine, sulfamethoxazole and sulfathiazole residues in milk. The simultaneous use of both bioassays identifies betalactam, tetracycline and sulfamide residues in milk. Neomycin, tylosin and lincomycin residues can also be detected, but these molecules are positive with the BT and BS bioassays, e.g., betalactams, given the microorganisms’ sensitivity to these molecules.