台灣健康成人攝取豆漿之異黃酮尿藥動力學   全文下載

Isoflavone Urine Kinetics after Giving Soymilk to Healthy Adults in Taiwan

本研究探討台灣健康成人攝取豆漿之異黃酮尿藥動力學。九位受試者攝取含200公克大豆之豆漿800毫升，收集0到48小時之分段尿液。各段尿液檢品以β-glucuronidase與sulfatase分別水解，利用HPLC分析?解前、後之尿液。結果顯示，尿液中無daidzin 、daidzein 、genistin 、genistein之原形存在，而daidzein 、genistein之glucuronides與sulfates為主要分子形態。另外，九位受試者之尿液皆檢出daidzein代謝物equol之glucuronides與sulfates。綜言之，台灣健康成人攝取豆漿之尿液中，主要排出daidzein 、 genistein及equol之glucuronides與sulfates。

This study was aimed to investigate the urine kinetics of isoflavones after oral intake of soymilk in healthy adults in Taiwan. Nine volunteers received 800 mL of soymilk equivalent to 200 g of soybeans and the urine were collected from 0 to 48 h. Daidzein, equol and genistein in urine were assayed by HPLC before and after hydrolysis with β-glucuronidase and sulfatase. The results revealed that the parent forms of daidzein and genistein were not detected in the urine with major forms being the glucuronides (G) and sulfates (S), mainly G. Besides, equol G and S, the metabolites of daidzein, were detected in the urine of all subjects. In conclusion, G and S of daidzein and genistein, mainly G, were excreted in the urine of healthy adults in Taiwan after ingestion of soymilk. In addition, G and S of equol were found in urine of all nine subjects.