花生四烯酸活化的血小板電子自旋共振系統評估抗氧化物   全文下載

Evaluation of Antioxidants Using an Arachidonic Acid-Stimulated Platelet Electron Spin Resonance System

電子自旋共振（ESR）是能直接地偵測出自由基的一個分光鏡技術，廣泛地被應用在抗氧化劑的自由基清除能力評估。以ESR和自由基誘捕劑方法，我們建立了一個新的含有細胞的分析系統。此一花生四烯酸活化血小板的電子自旋共振系統能夠產生氫氧自由基及碳中心－－自由基。應用這分析系統去評估飲食中抗氧劑的抗氧化活性包括resveratrol 、番茄紅素、五羥黃酮和芸香?。在被測試的化合物中resveratrol顯示最強的自由基清除活性。我們的結果在含細胞分析系統中提供有用的數據，建立天然飲食物質的抗氧化活性的評估模式。

A novel cell-containing assay system using electron spin resonance (ESR) and spin-trapping methods was established. We used this assay system to evaluate the antioxidant activities of various dietary antioxidants including resveratrol, lycopene, quercetin and rutin. Resveratrol showed the strongest radical-scavenging activity among the compounds tested. Our results provided useful data for the establishment of a model for evaluating the antioxidant activities of natural dietary substances in cell-containing systems.