離子液體作為添加劑毛細管區帶電泳法分析華北風毛菊中黃酮苷   全文下載

Analysis of Bioactive Flavonoid-O-glycosides in Saussurea mongolica Franch by Capillary Zone Electrophoresis Using Ionic Liquids as the Additive

本文中，我們採用1-烷基-3-甲基咪唑基離子液體作為毛細管電泳的添加劑分離了6種黃酮?。系統優化了離子液體烷基鏈，離子液體陰離子和離子液體濃度對分離的影響，結果表明，以含有5 mg/ml 1B-3mi-tFB的20 mm硼砂（pH值9.00）作為背景電解質，分離電壓為 15 kV，分離溫度為25°C時，分析物的分離效果最好。同時，本文初步考察了離子液體與分析物之間的作用機制。

In this study, a capillary zone electrophoretic method, in which 1-alkyl-3-methylimidazolium-based ionic liquids (IL) was used as the additive, was established to separate and identify six bioactive flavonoid-O-glycosides in Saussurea mongolica Franch. In order to investigate the traits of IL in capillary electrophoresis (CE), the influences of the alkyl group, the anionic part and the concentration of IL were investigated and discussed. Optimum separation was achieved with 20 mM borate buffer at pH 9.00 containing 5 mg/mL 1-ethal-3-methylimidazolium tetrafluoroborate. The applied voltage was 15 kV and the capillary temperature was kept constant at 25°C. The relative standard deviations (RSD) of the migration time and the peak area of each peak for six replicate injections were 0.75% - 1.45% and 3.18% - 3.91%, respectively. Moreover, it was worth noticing that the mechanism of interaction between IL and analytes was investigated preliminarily.