RP-HPLC/DAD 法測定烏拉爾甘草（Glycyrrhiza uralensis Fisch.）、光果甘草（G. glabra L.）中 5 個活性成分在生品與蜜炙品中的含量   全文下載

Quality Analysis of Raw and Honey-Processed Licorice of Glycyrrhiza uralensis Fisch. and G. glabra L. by Simultaneous Determination of Five Bioactive Components Using RP-HPLC/DAD Method

採用反相－－高效液相色譜法與二極管陣列檢測器聯用技術，建立同時測定甘草中5個生物活性成分—芹糖甘草?、甘草?、異甘草素－－葡萄糖芹糖?、異甘草?及甘草酸的檢測方法。該分析方法以乙?－－0.1%磷酸水溶液（v/v）為流動相梯度洗脫。 5個檢測成分分別在91.2 - 1824 μg/ml， 54.4 - 2720 μg/ml，6.4 - 512 μg/ml，6.4 - 512 μg/ml和20.4 - 2040 μg/ml範圍內呈現良好的線性關係（r2 ? 0.9998）；平均加樣回收率為95.86% - 103.11%。精密度、重複性以及準確性的考察均顯示該分析方法對以上5個成分有靈敏、準確的測定。應用建立的分析方法，對收集的42批烏拉爾甘草（Glycyrrhiza uralensis Fisch.）及光果甘草（G. glabra l.）的生品、蜜炙品中以上5個成分進行含量測定。結果顯示，兩種生甘草經蜜炙後，5個成分的含量均有所降低。烏拉爾甘草中甘草酸與甘草?含量之比在蜜炙前後無顯著變化，均高於5.0；光果甘草中這一比值在蜜炙前後亦無顯著變化，但低於3.0。由此認為甘草酸與甘草?含量之比可作為鑑別烏拉爾甘草與光果甘草的又一內在依據。

A reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed for simultaneous determination of five bioactive components, liquiritin apioside, liquiritin, licuraside, isoliquiritin and glycyrrhizin in licorice with diode-array detection (DAD). The method was performed with a mobile phase consisting of acetonitrile and 0.1% (v/v) phosphoric acid under gradient elution. All calibration curves of the five bioactive components showed excellent linearity (r2 ≥ 0.9997) within the ranges of 91.2 - 1824 μg/mL, 54.4 - 2720 μg/mL, 6.4 - 512 μg/mL, 6.4 - 512 μg/mL and 20.4 - 2040 μg/mL, respectively. The average recoveries ranged from 95.86% to 103.11%. This analytical method was also validated with respect to precision, repeatability and accuracy, and proved to be sensitive and accurate to simultaneously determine the five components in licorice. The developed method was further applied to quantify the contents of five components in forty two batches of either raw or honey-processed licorice samples of Glycyrrhiza uralensis Fisch. and G. glabra L. The results revealed that the contents of five components had descended in toto after the honey process. The content ratio of glycyrrhizin to liquiritin was more than 5.0 in licorice of G. glabra whereas it was less than 3.0 in that of G. uralensis in both raw and honey-processed licorice materials. This content ratio difference may be used to distinguish among licorice materials.