| 中文摘要 |
虎杖是在亞洲被廣泛使用的中草藥。本研究主要探討虎杖根乙醇萃取物是否能抑制eB病毒溶裂循環。細胞存活率以mtt [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide]方法測定；eB病毒溶裂循環期蛋白質的表現以流式細胞儀分析；以Real-time PCR方法分析病毒顆粒的釋放；轉染含有eB病毒溶裂循環期的極早期基因, BRlF1及BRlF1啟動子及報導基因質體至P3HR1循環細胞分析病毒溶裂循環極早期基因的轉錄。結果顯示虎杖根乙醇萃取物能抑制eB病毒極早期基因的轉錄，也可以抑制eB病毒溶裂期蛋白質的表現，包括Rta、zta及ea-d蛋白質的表現；進而也能降低eB病毒顆粒的產生。換言之，虎杖根乙醇萃取物有應用在避免eB病毒擴大增殖的價值。" |
| 英文摘要 |
Polygonum cuspidatum is widely used as a medicinal herb in Asia. In this study, we examined the ethanolic extract of Polygonum cuspidatum roots (PcE) for their capacity to inhibit the EBV lytic cycle. The cell viability was determined by the MTT [3-(4,5- dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide] method. The expression of Epstein-Barr virus (EBV) lytic proteins was analyzed by flow cytometric assay. Real-time quantitative PCR was used to measure the EBV virion production. Transient transfection analysis was subsequently performed to assess the transcription of lytic genes, including BRLF1 and BZLF1. Results showed that the PcE inhibited the transcription of EBV immediate early genes, the expression of EBV lytic proteins, including Rta, Zta, and EA-D and reduced the production of virus particles, showing that PcE is useful for preventing the proliferation of the virus. |
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