大鼠慢性鎘中毒實驗：合併給予維生素，氨基酸，抗氧化劑和必需金屬處置   全文下載

Chronic Cadmium Toxicity in Rats: Treatment with Combined Administration of Vitamins, Amino Acids, Antioxidants and Essential Metals

本研究以albino雄性大鼠為實驗動物，探討膳食營養素對鎘引起之肝，腎毒性的保護作用。大鼠隨機分為11組，對照組Group I給予口服生理食鹽水，Group II給予氯化鎘2 mg/kg皮下注射。其餘各組（Group III 、IV 、V 、VI 、VII 、VIII 、IX）給予10 mg/kg膳食營養素，分別為維生素B1，蛋氨酸，N-乙醯半胱胺酸（NAC），褪黑激素，鋅，維生素E，胱氨酸。 Group X給予0.1 mg/kg劑量的硒（Se），Group XI則合併給予NAC 、鋅，維生素B1和褪黑激素，所有的處理組均為口服三週，伴隨皮下注射鎘。鎘中毒大鼠肝，腎毒性表現於脂質過氧化程度和superoxide dismutase 、 glutathione peroxidase 、alkaline phosphatase 活性、總蛋白等數值明顯下降。相對於單獨給予各種膳食營養素，合併給予蛋氨酸，鋅，維生素B1和NAC，更能改善鎘引起的脂質過氧化和氧化壓力，因為顯著地啟動抗氧化防禦機制，因此對鎘引起的肝、腎毒性具保護作用。

The present study was designed to investigate the protective effect of dietary nutrients against cadmium-induced hepatic and renal toxicities in male albino rats. The animals were randomly divided into eleven groups where the control group (Group I) received physiological saline (p.o.) and Group II was administered with cadmium chloride (2 mg/kg, s.c.) only. Groups III, IV, V, VI, VII, VIII and IX received 10 mg/kg dietary nutrients, i.e. thiamine, methionine, N-acetylcysteine (NAC), melatonin, zinc (Zn), vitamin E and cystine, respectively. Group X received a dose of 0.1 mg/kg of selenium (Se) and Group XI was administered with a combination of NAC, Zn, vitamin B1 and melatonin. All treatments were administered orally for 21 days with a concomitant subcu-taneous (s.c.) cadmium treatment. Cadmium-intoxicated rats exhibited hepatic and renal toxicities as indicated by elevation of lipid peroxidation and marked decline in superoxide dismutase, glutathione peroxidase, alkaline phosphatase activities and total protein. However, a combined treatment with dietary nutrients, i.e. methionine, zinc, vitamin B1 and N-acetylcysteine ameliorated cadmium-induced lipid peroxidation and oxidative stress as they provoked the antioxidant defense system more significantly, when compared to each of them alone and hence is suggested as protective against cadmium-induced hepatic and renal toxicities.