雞血水解液抑制血管收縮素轉換酶   全文下載

Inhibition of Angiotensin I - Converting Enzymes by Enzymatic Hydrolysates from Chicken Blood

本研究至家禽屠宰工廠收集雞血，利用冷凍乾燥製成雞血粉，應用Alcalase 、Protease N和Prozyme 6三種蛋白水解?，分別以不同濃度（w/w，以蛋白質含量計算）在50°C下進行酵素水解，水解液再進行水溶性蛋白質含量、胜?含量、水解率和ACE抑制能力等分析探討。試驗結果顯示，酵素作用之水解液其可溶性蛋白質含量、胜?含量和水解率皆隨酵素濃度增加、水解時間越長而增加，雞血粉水解液在ACE抑制效果方面，以10% Alcalase水解5小時之條件為最高，10% Alcalase水解5小時具有抑制ACE活性之IC50值為0.34 mg peptide/mL，與其他各組相比顯著較佳。 10% Alcalase水解5小時雞血粉水解液經超過濾膜區分後，F3 區（< 3000 Da）的IC50值0.06 mg peptide/mL最低，具有最佳的抑制ACE活性。後續再將F3區劃分物以快速液相層析法分離，可得到五個波峰（P1、P2、P3、P4、 P5），其中P5（分子量470-190）具最強的ACE抑制活性，其有效抑制百分比 （IER, inhibitory efficiency ratio）為1071%/mg/mL，未來應有開發成為保健食品的潛力。"

This study examined the hydrolysis of chicken blood meal on soluble protein content, peptide content, the degree of hydrolysis and the inhibition of the Angiotensin I - Converting Enzyme (ACE). The results showed that soluble protein, peptide content and the degree of hydrolysis of the hydrolysates increased when either hydrolysis time or enzyme concentration increased. Five-hour long hydrolysis, using 10% Alcalase enzyme produced the highest ACE-inhibition activities. Under these conditions, the Alcalase IC50 value at 0.34 mg peptide/mL was significantly lower than that obtained from other combinations of enzyme, concentration and hydrolysis time. Separation of the hydrolysates by ultrafiltration isolated a fraction (F3) of less than 3000 Da molar mass. The F3 fraction performed with an IC50 value of only 0.06 mg peptide/mL. Further separation by FPLC using a Superdex peptide 10/300 GL gel column produced the highest inhibitory efficiency ratio (1071%/mg/mL). These results suggest that chicken blood hydrolysates can potentially be developed as functional food products in the future.