高氏柴胡製備之小柴胡湯對四氯化碳誘導大鼠急性肝損傷之保肝與抗氧化特性   全文下載

Hepatoprotective and Antioxidative Properties of Chinese Herbal Medicine Xiao-Chai-Hu-Tang Formulated with Bupleurum Kaoi Liu on Carbon Tetrachloride-Induced Acute Hepatotoxicity in Rats

柴胡（Bupleurum radix）是中草藥小柴胡湯（Xiao-Chai-Hu-Tang, XCHT）的主要材料，常用於治療肝炎。市面上有許多種類的柴胡，而高氏柴胡（Bupleurum kaoi Liu, Chao et Chuang）是台灣唯一的柴胡屬（Bupleurum）原生品種。本研究目的是探討以高氏柴胡製備之小柴胡湯的保肝能力與抗氧化特性。大鼠經過四氯化碳處理後，測定其天門冬胺酸轉胺?（aspartate aminotransferase, AST）、丙胺酸轉胺?（alanine aminotrans-ferase, ALT）、超氧歧化?（superoxide dismutase, SOD）、觸?（catalase）、麩胱甘?過氧化?（glutathione peroxidase, GPx）、麩胱甘?還原?（glutathi-one reductase, GRd）、麩胱甘?硫轉移?（glutathione S-transferase, GST）活性及脂質過氧化物含量，以評估小柴胡湯的保肝特性。結果顯示，預先管餵小柴胡湯萃取物再經過四氯化碳處理後的大鼠，其血清中上述酵素含量顯著低於只以四氯化碳處理的大鼠。丙二醛（malondialdehyde）的生成量在小柴胡湯萃取物合併四氯化碳處理組之大鼠也較四氯化碳組低；和小柴胡湯萃取物相較之下，高氏柴胡在保肝能力方面也具有同樣的效果。SOD 、 catalase 、GPx 、GRd和GST的活性在四氯化碳組呈現代償性地增加。由結果推測小柴胡湯或高氏柴胡水萃物可顯著地抑制脂質過氧化作用以及將抗氧化酵素活性回復至正常範圍而具有保肝功效。"

The Chinese herbal medicine, Xiao-Chai-Hu-Tang (XCHT), with Bupleurum radix as the main ingredient, is used for treating hepatitis. Although there are many varieties of Bupleurum in the herbal market, Bupleurum kaoi is the only indigenous species belonging to the Bupleurum genus plant in Taiwan which is commercially cultivated for making health-promoting products. The present study examined the hepatoprotective and antioxidative properties of XCHT formulated with commercially cultivated B. kaoi. The activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), glutathione reductase (GRd) and glutathione-S-transferase (GST) as well as lipid peroxidation in rats treated with CCl4 were analyzed to evaluate the hepatoprotective property of XCHT. The results showed that the enzyme levels in the serum of rats treated with XCHT extract prior to receiving CCl4 were significantly lower (p < 0.05) than those in the rats that received CCl4 only. Malondialdehyde formation was also reduced when XCHT extract was given. In comparison with the extract of XCHT, the extract of B. kaoi showed similar hepatoprotective abilities. Since the levels of SOD, catalase, GPx, GRd and GST were compensation increased in CCl4 treatment, it was suggested that XCHT or B. kaoi markedly suppressed lipid peroxidation and reversed the activities of the antioxidant enzymes to the normal levels.