中藥補養藥於人類周邊血液單核球細胞與人類單核白血球細胞株中細胞激素之調控分析   全文下載

Regulation of Cytokine Production by Treating with Chinese Tonic Herbs in Human Peripheral Blood Mononuclear and Human Acute Monocytic Leukemia Cells

中藥的補養藥常以非傳統療法方式用於抗癌或免疫相關等疾病，而細胞激素在免疫系統內扮演著重要的角色。本研究乃針對常用的補養藥，利用人類周邊血液單核球細胞及人類單核白血球細胞株，進行細胞素調控作用之探討。十五種選定的中藥材，其於植物血球凝集素A誘導人類周邊血液單核球細胞試驗中，芍藥具有抑制增殖的作用且亦能誘導干擾素 γ 的生成。於人類單核白血球細胞株試驗中，利用多重標的偵測技術，同時分析17種細胞激素，並以反轉錄聚合?連鎖反應（PCR）／定量即時功能聚合?反應（q RT-PCR）來評估其基因之表現。結果顯示菟絲子具誘發第8介白質及巨噬細胞發炎蛋白-1β 之釋放；山藥、土肉桂和甘草均能刺激嗜中性白血球促進因子之釋放，同時亦影響其訊息核醣核酸mRNA的轉錄作用。由本實驗之結果，不同的補養藥運用在人類不同的細胞上具多樣性的免疫調節作用，可透過細胞激素或其他因子進行免疫調節而成為人體免疫調節能力的輔助用藥。本研究首次揭露利用多重標的偵測技術高效能地分析中藥補養藥萃取物對於細胞激素釋放調節作用之可行性。

Chinese tonic herbs are often used as remedies for the treatment of cancer or immunity related diseases. Cytokine regulation is one of the pathways that modulates the immune system. In the present study, we investigated the effects of commonly used Chinese herbs on cytokine regulation in human peripheral blood mononuclear cells (PBMC) and human acute monocytic leukemia cell line, THP-1. Among the 15 selected Chinese herbs, Paeonia lactiflora exhibited inhibitory effect on phytohemagglutinin (PHA)-acti-vated PBMC proliferation and induced IFN-γ production. We also used a multiplexed flow cytometric bead-based assay to analyze the release of 17 cytokines and reverse transcription polymerase chain reaction (RT-PCR)/quantitative real-time PCR (qRT-PCR) to evaluate gene expression. Cuscuta chinensis induced both interleukin-8 and MIP-1β release in THP-1 cells and influenced mRNA transcription. Dioscorea opposita, Cinnamomum osmophloeum, and Glycyrrhiza uralensis enhanced secretion of granulocyte-macrophage colony-stimulating factor (GM-CSF) and influenced GM-CSF gene expression. As such, different Chinese tonic herbs may regulate immunomodulatory functions in human cells via diverse processes such as the modulation of different cytokines and may be used as adjuvant immunity-boosting drugs. The present study is the first to use high-throughput multiplexed flow cyto-metric method to analyze cytokine regulatory effects of herbal extracts.