馬鹿茸（Cervus elaphus Linnaeus）水提取物分離成分之抗氧化活性研究   全文下載

Antioxidant Activity of Aqueous Extract Fractions of Velvet Antler (Cervus elaphus Linnaeus)

鹿茸（Velvet Antler）具有強健體魄，免疫調節和抗衰老等作用，常用於功能性食品和保健品。本研究採用DPPH 、FRAP 、亞鐵螯合以及抑制亞油酸自氧化等方法測定馬鹿茸水提取物（AEVA）之抗氧化活性。研究發現，AEVA在上述四種方法中均表現出抗氧化活性。去除蛋白質後， AEVA之抗氧化活性顯著提高。經半製備反相高效液相層析法對去蛋白成分（AEVA-S）進一步分離製備。採用LC/MS，HPLC及UV/Vis等儀器分析方法對上述活性最強的分離成分（AEVA-SII）進行鑑定。結果顯示， HPLC譜圖中的5個主要色譜峰分別為核?酸（3′-CMP，2′-CMP，3′-UMP和2′-UMP）和次黃嘌呤。AEVA-SII中之核?酸無自由基清除能力和鐵還原能力。然而，僅UMP表現出亞鐵螯合能力，占AEVA-SII總螯合能力的34.75%，上述結果表明，AEVA-SII中還存在其他未鑑定出之具有較強抗氧化活性的成分。

Velvet antler is believed to have body strengthening, immunomodulatory and anti-aging effects. It is used in Chinese commercial functional foods and nutraceuticals. The antioxidant activity of the aqueous extract of velvet antler (AEVA) from Cervus elaphus Linnaeus was evaluated with DPPH-radical scavenging, FRAP, Fe2+-chelating and inhibition of linoleic acid autoxi-dation assays. AEVA showed antioxidant activity in all four assays. After removal of protein from AEVA, the antioxidant activity was significantly elevated. A semi-preparative HPLC equipped with a C18 column was used for further separation of the non-pro-tein components (AEVA-S). Identification of the most active fraction (AEVA-SII) of AEVA-S was accomplished by LC/MS, HPLC and UV/Vis analyses. The HPLC chromatogram showed five main peaks identified as nucleotides (3′-CMP, 2′-CMP, 3′-UMP and 2′-UMP) and hypoxanthine. Nucleotides in AEVA-SII exhibited no free-radical scavenging and ferric-reducing activity. Only UMP exhibited Fe2+-chelating activity which accounted for 34.75% of the total Fe2+-chelating activity of AEVA-SII. The results indicated that other unidentified components with antioxidant activity were present in AEVA-SII.