| 中文摘要 |
白及（Rhizoma Bletillae）為傳統常用的中藥材，而原生之台灣白及（Bletilla formosana）分佈於全島各地，本研究收集屏東縣霧台鄉、花蓮縣秀林鄉、宜蘭縣大同鄉（B. formosana (Hayata) Schltr.）、台東縣蘭嶼鄉（B. formosana (Hayata) Schltr. f. kotoensis (Hayata) T. P. Lin）等不同地區之野生白及，以militarine 、cinnamic acid 、1,8-bi(4-hydroxybenzyl)-4-methoxyphen-anthrene-2,7-diol（BHMD）和4,7-dihydroxy-1-p-hydroxybenzyl-2-methoxy-9,10-dihydro-phenanthrene（DHMD）等四種指標成分進行HPLC定量分析，並以Folin-Ciocalteu方法測定總酚類含量，檢測DPPH清除能力作為其抗氧化性評估方法。研究結果顯示，不同地區蒐集的野生白及，cinnamic acid 和 militarine成分在塊莖中含量以宜蘭地區較高，而台東地區之蘭嶼白及中BHMD及DHMD在塊莖含量最高，且實生苗成分含量略低於母株。結果亦顯示台東地區的蘭嶼白及其總酚含量較高，且具有較高的清除DPPH 的能力。" |
| 英文摘要 |
Bai ji (Rhizoma Bletillae) is a well known traditional Chinese medicine, and Taiwan bai ji (Bletilla formosana (Hayaata) Schltr.) is commonly available in Taiwan. In this study, B. formosana (Hayata) Schltr. was collected from Wutai township (Pingtung county), Shaolin township (Hualien county) and Datung township (Yilan county), while B. formosana (Hayata) Schltr. f. kotoensis (Hayata) T. P. Lin was collected from Orchid Island (Taitung county). Four components, namely militarine, cinnamic acid, 1,8-bi(4-hydroxybenzyl)-4-methoxyphenanthrene-2,7-diol (BHMD), and 4,7-dihydroxy-1-p-hydroxybenzyl-2-methoxy-9,10-dihydro-phen-anthrene (DHMD), were used as marker compounds for quantitative analysis by HPLC. The Folin-Ciocalteu method was used to measure the total phenolic content in extracts. DPPH radical-scavenging assay was employed to measure antioxidant activity. Our results showed that the contents of cinnamic acid and militarine in the tubers from Yilan were higher than those in other regions. Moreover, the contents of BHMD and DHMD in the tuber of the plants from Orchid Island were higher than those in other regions. In addition, the content of the components in the seedlings grown by tissue culture were lower. B. formosana (Hayata) Schltr. f. kotoensis (Hayata) T. P. Lin from Taitung had a higher total phenolic content and higher DPPH scavenging ability. |
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