保健食品中三種含硫威而鋼類緣物之純化與鑑定   全文下載

Isolation and Identification of Three Thio-sildenafil Analogues in Dietary Supplements

三種暗示具壯陽效果之保健食品，分別檢出含硫威而鋼類緣物（Thio-sildenafil analogues）成分，並透過核磁共振儀（NMR）、高解析質譜儀（HRMS）、紅外線光譜儀（IR）、紫外光分光光度法（UV）及高效液相串聯式質譜儀（LC/MS/MS）等方法鑑定其結構。這些類緣物包括thiosilde-nafil 、thiohomosildenafil及hydroxythiohomosildenafil；與威而鋼主成分（sildenafil）比較，sildenafil雜環上羧基之氧原子已由硫取代，因其主結構與sildenafil相似，可能引起不良副作用，為保障民眾健康，臺灣已將此等成分列入非法摻加西藥例行檢查項目。截至2009年10月為止，本實驗室計檢出thiosildenafil 6次、thiohomosildenafil 4次及hydroxythiohomosildenafil 3次。

Three compounds were found to have been illegally added to dietary supplements marketed for erectile dysfunction. The struc-tures of these compounds were identified with NMR, HRMS, IR, UV and LC/MS/MS, after comparison with the structure of silde-nafil, data showed that the oxygen atom of the carbonyl group in the heterocyclic ring had been replaced with a sulfur atom, these thio-sildenafil analogues were identified as thiosildenafil, thiohomosildenafil and hydroxythiohomosildenafil. Since their struc-tures were similar to that of sildenafil, according to structure-activity relationship, the side effects of these analogues might also associate with that of sildenafil. In accordance with this finding, thio-sildenafil analogues have been included in the inspection list of illegal adulterants in Taiwan. From January to October 2009, thiosildenafil was detected in 6 samples, thiohomosildenafil was detected in 4 samples and hydroxythiohomosildenafil was detected in 3 samples.