體外試驗評估乳酸菌抗癌細胞增生及其抗氧化能力   全文下載

In Vitro Effects of Lactic Acid Bacteria on Cancer Cell Viability and Antioxidant Activity

本研究利用兩株由台灣本土分離之乳酸菌株（Lactobacillus paracasei ssp. paracasei NTU 101 及 L. plantarum NTU 102）及十株乳酸菌株以體外試驗評估其抑制癌細胞及提高抗氧化之能力。研究結果顯示 Lactobacillus acidophilus BCRC14079 熱不活化細胞（heat-killed cell, HKC）具有最高之總抗氧化之活性（82.3%）。清除1,1-diphenyl-2-picryl-hydrazyl（DPPH）自由基活性部份，除了L. salivarius ssp. salivarius BCRC 14759 及 Bifidobacterium breve BCRC11846 之 HKC 無顯著活性外，其餘乳酸菌樣品呈現之清除活性約為 14.8-32.9%。還原力（reducing activity）部份，L. acidophilus BCRC 14079之細胞質區分（cytoplasmic fraction, CF）相較於B. adolescentis BCRC 14606之 HKC（全部樣品中活性最低）有明顯的增加（約6倍）。細胞存活率部份，不同的乳酸菌株對於抑制癌細胞生長皆有不同的影響。利用本土分離乳酸菌株 L. paracasei ssp. paracasei NTU 101 及 L. plantarum NTU 102 處理乳癌細胞株（MDA-MB-231），並以流式細胞儀分析該細胞週期，結果顯示可有效抑制乳癌細胞停滯於G0/G1期間。上述結果顯示本土乳酸菌株具有強的抗氧化能力及抑制癌細胞生長能力。"

We used two local Lactobacillus strains (L. paracasei ssp. paracasei NTU 101 and L. plantarum NTU 102) and other 10 lactic acid bacteria (LAB) strains from the Biosource Collection and Research Center (BCRC) to evaluate the health promoting capa-bility including inhibitory effects on cancer cell lines and antioxidant activities in vitro. The heat-killed cells (HKC) of Lactoba-cillus acidophilus BCRC14079 have the highest total antioxidative activity (82.3%). 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging effects were in the range of 14.8-32.9%, except for the HKC of L. salivarius ssp. salivarius BCRC 14759 and Bifido-bacterium breve BCRC 11846. Twelve strains showed varying degrees of reducing activity. The reducing activity of cytoplasmic fraction (CF) of L. acidophilus BCRC 14079 was found to be significantly more pronounced (six-fold) than that of the HKC of B. adolescentis BCRC 14606, which was the lowest of all. In the cell viability, the inhibitory effects on cancer cells were very different which depended on various LAB strains. Flow cytometry study on human breast adenocarcinoma cells (MDA-MB-231) treated with local lactobacilli strains (L. paracasei ssp. paracasei NTU 101 and L. plantarum NTU 102) isolated in Taiwan showed the highest percentage of arrested at G0/G1 phase in the cell cycle. These results show that local lactobacilli strains have strong antioxidative and anticancer activities.