土耳其酒中之赭麴毒素A（Ochratoxin A, OTA）含量   全文下載

Determination of Ochratoxin A in Turkish Wines

本研究以一經確效、簡單且靈敏度高的分析方法，檢測土耳其產製酒類中的赭麴毒素（orchratoxin A, OTA）含量。高效液相層析儀配合螢光檢測法檢測25種酒樣品中OTA含量。以等位沖提模式配合逆相C18層析管柱離析OTA和氟苯水楊酸（內部標準品），移動相為乙?：水：醋酸（50 : 48 : 2, v/v/v），流速0.4 mL/min ，激發波長350 nm，發射波長450 nm，平均檢測時間為13分鐘。酒類檢體未經萃取或濃縮，僅以0.2 μm濾膜簡單過濾，即直接注入儀器分析，OTA在μg/L含量可由層析法檢測，。檢驗結果，25件檢體中有14件的OTA含量超過許可標準（< 2 μg/L），OTA含量在紅酒中較高。"

A validated, simple and highly sensitive analytical method was described for the determination of Ochratoxin A (OTA) in various wines produced in Turkey. OTA concentrations of 25 wine samples were examined using high performance liquid chromatography coupled with fluorescence detection. OTA and diflunisal (internal standard) were separated using isocratic elution mode with a reversed phase Nucleosil® C18 column. The mobile phase consisted of CH3CN : H2O : CH3COOH (50 : 48 : 2, v/v/v) was pumped at a flow rate of 0.4 mL/min. The analytes were detected at 330 nm excitation and 450 nm emission wavelengths within an average time of 13 min. Samples were prepared by simply filtrating the wine samples through a 0.2 μm filter and injected into the system without further extraction or concentration steps. OTA was detected in μg/L levels with adequate chromatographic resolution. It was found that the amount of OTA was higher than the permitted limits (< 2 μg/L) in 14 out of 25 samples, especially in red wines.