綠茶多酚分子 [(-)-Epigallocatechin-3-gallate] 在大鼠半月板纖維軟骨細胞受貝它型介白質素1 (IL-1β)刺激後對第二型環氧化酶 (Cyclooxygenase 2) 及基質金屬蛋白酶 (Matrix metalloproteinase) 3、9、13基因之效用   全文下載

Effects of (-)-Epigallocatechin-3-gallate on Cyclooxygenase 2 and Matrix Metalloproteinase 3, 9, 13 Expression Induced by IL-1ß in Rat Meniscal Fibrochondrocytes

本研究目的是在探討綠茶多酚分子在大鼠正常的半月板纖維軟骨細胞受貝它型介白質素1 刺激後，對軟骨細胞第二型環氧化?及基質金屬蛋白?3 、9 、13基因之效用。從正常老鼠後腳膝關節取得半月板軟骨組織，利用膠原蛋白酵素將組織分解，分離出軟骨細胞進行初級培養。將初級培養的軟骨細胞先加入50 μ m的綠茶多酚分子12小時，再加入貝它型介白質素1刺激6 小時，之後抽取核醣核酸，分別利用反轉錄聚合?鏈連鎖反應及即時定量核酸聚合?連鎖反應來評估第二型環氧化?及基質金屬蛋白?3 、9 、13基因表現變化。結果發現藉著貝它型介白質素1 刺激初級培養的軟骨細胞第二型環氧化?及基質金屬蛋白?3 、9 、13基因有被誘導表現，而加入綠茶多酚分子一同培養有被抑制，具有統計學之意義（p < 0.05）。由此細胞實驗之結果可得知綠茶多酚分子可能可以應用在治療半月板軟骨發炎併有細胞激素貝它型介白質素1 之分泌。

The objective of this study was to examine the effect of (-)-epigallocatechin-3-gallate (EGCG) on cyclooxygenase 2 (COX-2) and matrix metalloproteinase (MMP) 3, 9, and 13 expressions induced by IL-1b in rat meniscal fibrochondrocytes. Rat normal meniscal fibrochondrocytes were enzymatically isolated from meniscal tissue from the hind leg knee joint. Cells were pretreated with EGCG at a concentration of 50 mM to examine its effect in the presence of IL-1b insult. Reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time PCR were used to assess the COX-2 and MMP3, 9, and 13 gene expressions, respectively. COX-2 gene upregulation induced by IL-1b in primary cultures of rat meniscal fibrochondrocytes was significantly suppressed by EGCG (p < 0.05). MMP 3, 9, and 13 gene expressions were also significantly induced by IL-1b stimulation and significantly inhibited by EGCG treatment (p < 0.05). These results indicated that EGCG may be of value in the treatment of meniscal inflammation associated with IL-1b secretion.