應用鹼性高錳酸鉀之動力學光度法分析草酸萘H胺（Naftidrofuryl Oxalate）及長春胺（Vincamine）製劑   全文下載

Kinetic Spectrophotometric Analysis of Naftidrofuryl Oxalate and Vincamine in Pharmaceutical Preparations Using Alkaline Potassium Permanganate

本研究係以簡單、快速且靈敏的動力學光度法，分析草酸??胺（NF）和長春胺（VN）及其製劑。以動力學光度法分析受試藥物在高錳酸鉀中的氧化作用，於吸光值610 nm處測得錳酸鹽氧化物；影響顏色產生的變數及實驗條件皆已最適化。受試藥物濃度採固定時間法（fixed-time method）之回歸方程式（regression equation）計算。雖然，固定吸光光度法（fixed absorbance method）和速率常數法（rate constant method）也可測定此兩種藥物，但固定時間法比較合適。本方法的可信度和分析效能，包括線性關係、分析範圍、精密度、準確度、偵測及定量極限，均經統計學確效。草酸??胺和長春胺濃度範圍分別為3-15 μg/mL及4-14 μg/mL時，校正曲線圖具線性相關。以本開發方法分析草酸??胺和長春胺及其製劑可得到滿意結果，且其假設之反應途徑亦被提出。

A simple, rapid and sensitive kinetic spectrophotometric method is described for the analysis of naftidrofuryl oxalate (NF) and vincamine (VN) in pure form and in their pharmaceutical preparations. The procedure was based on the kinetic investigation of the oxidation of the studied drugs with alkaline potassium permanganate and the absorbance of the produced manganate species was measured at 610 nm. Variables affecting the color development were investigated and the conditions were optimized. The concentration of the studied drugs was calculated using the regression equations for the fixed-time method. The determination of the two drugs by fixed absorbance and rate constant methods was possible but the fixed time method was more applicable. The reliability and analytical performance of the proposed method including linearity, ranges, precision, accuracy, detection and quantification limits were statistically validated. Calibration graphs are linear over the concentration ranges of 3-15 μg/mL and 4-14 μg/mL for NF and VN, respectively. The proposed method was satisfactorily applied for analysis of pharmaceutical preparations containing the studied drugs. A proposal of the reaction pathway was postulated.