利用實驗設計及高效液相層析法探討芫荽中 Quercetin Kaempferol 之最佳萃取條件   全文下載

Investigation of Optimum Extraction Conditions for Determination of Quercetin and Kaempferol in Coriander (Coriundrum sativum L.) by Using Experimental Design and HPLC

本研究利用實驗設計及高效液相層析法，探討分析芫荽中 quercetin 及kaempfero 時的最佳萃取條件。萃取過程中，影響實驗的 5 個因子包括萃取溶劑中甲醇百分比、萃取溶劑體積、水解作用之鹽酸濃度、萃取時間和溫度。本研究採中央組合設計探討這些因子對萃取回收的影響，並以格點搜尋法找出最佳實驗條件。研究結果顯示，最佳萃取條件為以含有 1.85 M HCl 之 45% 甲醇溶液 30 mL，於 84°C 下迴流 2 小時；在此條件下，分析quercetin 及 kaempferol 時的重複性，相對標準偏差為 3.6% 及 4.0%，回收率為 96% 及 94%；quercetin 與 kaempferol 之檢出限量、線性範圍、同日內與異日間相對標準偏差分別是0.19、0.18；0.19-48.0；0.18-30.0 μg/mL （R2 = 0.99） 及 3.0% - 4.0%。芫荽中 quercetin 與 kaempferol 濃度分別是 23.1 與10.1 mg/Kg。

Optimum conditions for extraction of quercetin and kaempferol from coriander are determined using experimental design and HPLC method. Effects of five experimental factors, including percentage of methanol in aqueous extraction solvent, volume of extraction solvent, concentration of HCl used for hydrolysis, extraction time, and temperature on the extraction recovery, are investigated. Central composite design is used to investigate the effects of these factors and grid search method is used to find the optimum conditions. The optimum conditions are 30 mL of 45% aqueous methanol, containing 1.85 M HCl, refluxed for 2 hours at 84°C. The extraction procedures at optimum conditions for quercetin and kaempferol show good repeatability with relative standard deviations of 3.6%, 4.0% and recoveries of about 96%, 94%, respectively. Limit of detection (LOD), Linear dynamic range, intra-day and inter-day R.S.D for quercetin and kaempferol are 0.19, 0.18, 0.19-48.0, 0.18-30.0 μg/mL (R2 = 0.99), and 3.0% - 4.0% respectively. Concentrations of quercetin and kaempferol in Coriander are found to be 23.1 and 10.1 mg/Kg respectively.