馬鹿茸水提物及其模擬胃腸消化物體外對免疫細胞的作用   全文下載

Immunomodulatory Effects of Aqueous Extract of Velvet Antler (Cervus elaphus Linnaeus) and Its Simulated Gastrointestinal Digests on Immune Cells In Vitro

鹿茸是中國傳統中藥，具有強壯機體，免疫調節和抗衰老等作用。鹿茸常用於功能性食品和保健品，本研究通過小鼠脾淋巴細胞增殖實驗和腹腔巨噬細胞吞噬中性紅實驗及 NO 的產生，探討馬鹿茸水提物（AEVA）及其模擬胃腸消化物（SGDs）的免疫調節作用。在體外，AEVA（10-400 μg/mL）對 ConA 誘導的脾淋巴細胞增殖有顯著的抑制作用，但對 LPS 誘導的脾淋巴細胞增殖無作用。AEVA 對巨噬細胞吞噬中性紅也有抑制作用。在模擬胃腸消化的過程中，AEVA 始終保持對 ConA 誘導的脾淋巴細胞增殖的抑制作用，並有一定程度的增強。然而，在此過程中，對巨噬細胞吞噬中性紅的抑制作用減弱。此外，單獨使用 AEVA 對巨噬細胞產生 NO 沒有影響，SGDs（200 μg/mL）對 LPS 誘導的巨噬細胞產生 NO 有促進作用。上述結果表明，AEVA 經胃腸消化後仍然保持著對 T 細胞的抑制作用，而對巨噬細胞功能的作用卻發生了變化。

Velvet antler, a traditional Chinese medicine, is believed to have body-strengthening, immunomodulatory and anti-aging effects. It has been used in Chinese commercial functional foods and nutraceuticals. This study aimed to investigate the immunomodulatory effects of aqueous extract of velvet antler (AEVA) from Cervus elaphus Linnaeus and its simulated gastrointestinal digests (SGDs) in vitro. Experiments were carried out by measuring the effects of AEVA and SGDs on splenocyte proliferation, neutral red pinocytosis and nitric oxide (NO) production by macrophages. AEVA (10-400 μg/mL) showed inhibitory effects on concanavalin A (ConA)-stimulated splenocyte proliferation while no difference was observed when lipopolisaccharide (LPS) was used as stimulus. An inhibitory effect was also observed in neutral red pinocytosis by AEVA treated macrophages. During simulated gastrointestinal digestion, AEVA kept and strengthened its inhibitory effect on ConA-stimulated splenocyte proliferation. However, digestion of AEVA counteracted the inhibitory effect on pinocytosis. In addition, AEVA alone had no effect on NO production by macrophages while some SGDs (200 μg/mL) increased NO production by LPS-stimulated macrophages. The results indicated that AEVA could keep its inhibitory effect on T-cells by oral administration. In contrast, the effect of AEVA on macrophage function was changed during digestion.