利用快速分流注射光譜儀分析四環黴素、氯黴素及羥四環黴素   全文下載

A Rapid Flow Injection Spectrophotometric Analysis for Tetracycline Chlortetracycline or Oxytetracycline

快速分流注射（FI）光譜儀可偵測四環黴素、氯黴素或羥四環黴素。將150 μL 標準品或樣品溶液注入含三價鐵的試劑中（500 μg/mL, 5.0×10-3 mol/L 硝酸）其最適流速為4.0 mL/min。三價鐵之四環黴素複合體可在423 nm 被測得。分流注射系統及實驗條件的最適化依實驗方法而改變。在最適條件下，由曲線圖得到之結果為1.0 - 100.0 μg/mL，及其各藥物之偵測極限（s/n = 3）為0.1 μg/mL。TC、CTC、OTC 相關係數分別為0.995、0.994 和0.990。此方法可成功運用在測定這些藥物的生產量及配藥上。"

A rapid flow injection (FI) spectrophotometric procedure for the determination of tetracycline(TC), chlortetracycline(CTC) or oxytetracycline(OTC) has been developed. The method is based on the injection of 150 μL standard or sample solution into a reagent stream of iron (III) (500 μg/mL in 5.0 × 10-3 mol/L HNO3) with the optimum flow rate of 4.0 mL/min. The brown iron (III)- tetracycline complex is monitored at 423 nm. The flow injection system and the experimental conditions were optimized by means of univariate method. Under the optimum conditions, calibration graphs were obtained for 1.0 - 100.0 μg/mL and the detection limits (s/n = 3) were 0.1 μg/mL of each drug. The correlation coefficients were 0.995, 0.994 and 0.990 for tetracycline, chlortetracycline and oxytetracycline, respectively. The method was successfully applied to the determination of these drugs in pharmaceutical formulations with a sample throughput of 70/h.