以 β-環糊精聚合物作為支持體直接光度法測定清涼飲料中的檸檬黃   全文下載

Direct Solid-phase Spectrophotometric Determination of Tartrazine in Soft Drinks Using β-Cyclodextrin Polymer as Support

本文報導了一個靈敏的固體相光度法測定清涼飲料中微量檸檬黃的新方法。在這個方法中，所利用的β- 環糊精聚合物（60-80 目）和離子交換樹脂相比，在紫外和可見光區吸收很小，其作為固體相的支持體，並用於從樣品溶液中分離收集檸檬黃，然後在435 nm 波長下直接用於測定。檸檬黃的最佳分離條件是pH 1.0、20°C、反應時間40 min。所提出的方法用於清涼飲料中檸檬黃的測定，結果滿意。

A sensitive and new solid-phase spectrophotometry for the determination of tartrazine in soft drinks has been developed. In this method, β-cyclodextrin epichlorohydrin polymer (β-CDP) of 60-80 mesh, which has less background absorbance in ultraviolet and visible region compared to other ion-exchanger resins, was used to support direct adsorption of tartrazine from aqueous solutions to form a solid complex (tartrazine-β-CDP complex). The effects of pH, temperature, time and ionic strength on the β- CDP including tartrazine were determined. The optimum conditions were obtained (pH, 1.0; temperature, 20°C; time, 40 min). The method was applied for the direct solid phase spectrophotometric determination of tartrazine in soft drink samples at the maximum absorption wavelength of 435 nm with satisfactory results.