| 中文摘要 |
食品及清涼飲料中常見的七種化學合成色素（amaranth, ponceau 4r, sudan red 1, tartrazine, sunset yellow FCF, fast green FCF and brilliant blue FCF）以高效能液相層析法使用C18 單一管柱來進行研究。移動相緩衝溶液組成使用含有甲醇的醋酸鹽溶液，調整不同的pH 值得到最佳化的波長測定，以滯留時間的相對標準偏差、波峰區域和峰高值判定結果分別為0.18-0.83， 0.44-3.49 及0.49-3.56%。其amaranth, ponceau 4r，sudan red 1，tartrazine，sunset yellow FCF，fast green FCF and brilliant blue FCF 的偵測極限（at a signal-to-noise ratio of 3）分別為3.38， 2.89， 2.62， 1.92， 3.38，1.23 與1.56 μg/L。這個最佳化方法可以被應用在含有這七種合成色素的食品及四種非酒精性飲料之鑑定及偵測。結果顯示，在泰國有一些含有比法定規定更高濃度的合成色素被添加於食品和非酒精性飲料中。" |
| 英文摘要 |
Seven synthetic dyes (amaranth, ponceau 4R, sudan red 1, tartrazine, sunset yellow FCF, fast green FCF and brilliant blue FCF), commonly used as colorant in foodstuffs and soft drinks, were investigated on monolithic C18 column by high performance liquid chromatography. The compositions of mobile phase containing methanol-water in acetate buffer at different pH were optimized at the maximum detection wavelength of each synthetic dye. Relative standard deviations of retention time, peak area and peak height for seven synthetic dyes were 0.18-0.83, 0.44-3.49 and 0.49-3.56%, respectively. The detection limit (at a signal-to-noise ratio of 3) for amaranth, ponceau 4R, sudan red 1, tartrazine, sunset yellow FCF, fast green FCF and brilliant blue FCF were 3.38, 2.89, 2.62, 1.92, 3.38, 1.23 and 1.56 μg/L, respectively. The optimal methods were applied to identify and determine the synthetic dyes in seven foodstuffs and four soft drinks. The results showed that some samples contained synthetic dye at a higher concentration than the maximum legal limit in foods and soft drinks of Thailand. |
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