以醣類分析鑑別燕窩真偽   全文下載

Authentic Determination of Bird’s Nests by Saccharides Profile

本研究探討以高效離子液相層析儀- 脈衝式電流檢出器（high performance ion liquid chromatography with pulsed amperometric detector, HPAEPAD）分析燕窩中醣類的組成以鑑別燕窩真偽之方法，燕窩中醣蛋白之醣類組成為岩藻糖（fucose, Fuc）0.44%，鼠李糖（rhamnose, Rha）0.20%，N-半乳糖胺（galactosamine, GalN）4.19%，N- 葡萄糖胺（glucosamine, GlcN）5.29%，半乳糖（galactose, Gal）5.03%，甘露糖（mannose, Man）0.75% 及N- 乙醯神經胺酸（N-acetylneuraminic acid, NeuAc）10.80% ± 0.76。本研究利用唾液酸估算冰糖燕窩產品中燕窩的含量，以Neu5Ac/GalN 比值判斷冰糖燕窩產品的品質。市售乾品燕窩之Neu5Ac/GalN 比為2.3-3.0， 20 件市售冰糖燕窩產品之Neu5Ac/GalN 比為0.03-3.0。"

A simple high performance ion liquid chromatography with pulsed amperometric detector (HPAE-PAD) was applied to the saccharide profile analysis for the authenticity identification of edible bird’s nests. The glycoproteins in authentic edible bird’s nests, as reference materials for this study, contain fucose (Fuc) 0.44%, rhamnose (Rha) 0.20%, galactosamine (GalN) 4.19%, glucosamine (GlcN) 5.29%, galactose (Gal) 5.03%, mannose (Man) 0.75% and N-acetylneuraminic acid (NeuAc) 10.8% ± 0.76. The NeuAc content was used as an index to estimate the content of authentic bird’s nest contained in dried powder. The dried powder was prepared by using bird’s nests obtained from retail stores, or from the gel substance of bottled products of bird’s nest steeped in rock sugar solution (BNsrss). The quality of dried bird’s nest was also judged by the NeuAc/GalN ratio. The NeuAc/GalN ratios in commercial products claimed as edible dried bird’s nest ranged from 2.3 to 3.0, while those in dried powders from gel substance claimed as BNsrss ranged from 0.03 to 3.0.