牛磺酸對於老鼠攝食氧化膽固醇和氧化魚油所產生的毒性作用影響   全文下載

Effect of Taurine on Toxicity of Oxidized Cholesterol and Oxidized Fish Oil in Rats

多數水產品中含有高量膽固醇及高度不飽和脂肪酸，此外亦含有牛磺酸。在水產品利用方面除生鮮食用外，其中也有不少水產品會在加工後加以利用，由於膽固醇的氧化和油脂氧化非常的相似。所以產品在加工處理後產生膽固醇氧化及油脂的氧化，是無可避免的。本研究針對攝食食品中含有氧化膽固醇和氧化魚油所產生的毒性影響及添加牛磺酸後對雄性Wistar老鼠之影響，以30 隻老鼠分成5 大組，評估餵食添加氧化魚油（3%）和膽固醇（2%）、牛磺酸（5%）添加到氧化魚油（3%）和膽固醇（2%）、氧化魚油（3%）和氧化膽固醇（2%）及添加牛磺酸到氧化魚油（3%）和氧化膽固醇（2%）對於老鼠攝食之影響，分別飼養6 週，並且分析老鼠血液中之生化指標等，實驗發現在餵食牛磺酸可以增加在血液中的 high density lipoprotein-cholesterol （HDL-C），血液中的 glutathione （GSH），降低老鼠血液中的 alkaline phosphatase （ALP）、alanine transaminase （ALT）、aspartate transaminase （AST）、low density lipoprotein-cholesterol （LDL-C）、very low density lipoprotein-cholesterol （VLDL-C）、triglyceride、total cholesterol 和肝體比及肝臟中的thiobarbituric acid-reactive substances （TBARS），由此顯示食物中添加牛磺酸在老鼠的飲食中可預防和降低氧化膽固醇和氧化魚油所產生的毒性作用，並扮演重要的角色。

Taurine (2-aminoethanesulfonic acid) occurs naturally in food, especially in seafood and meat. The aim of our investigation was to evaluate the effect of dietary taurine on oxidized cholesterol and oxidized fish oil induced toxicity in male Wistar rats. Thirty male wistar rats were fed with diets supplemented with 5% taurine, 2% oxidized cholesterol or 3% oxidized fish oil for 6 weeks. After feeding such diet, taurine could increase the high density lipoprotein-cholesterol (HDL-C) level in plasma, and glutathione (GSH) level in plasma, and decrease the activities of alkaline phosphatase (ALP), alanine transaminase (ALT), aspartate transaminase (AST) in plasma, and the levels of low density lipoprotein-cholesterol (LDL-C), very low density lipoprotein-cholesterol (VLDL-C), triglyceride, total cholesterol in plasma, and relative ratios of liver weight to body weight and thiobarbituric acid-reactive substances (TBARS) level in the rat liver caused by oxidized cholesterol and oxidized fish oil. It could reduce the biochemical parameters characteristic in the plasma and rate liver caused by oxidized cholesterol and oxidized fish oil. It was also found that taurine possessed a good recovering effect and a short-term preventing effect from the toxicity of oxidized cholesterol and oxidized fish oil in rats. The results suggest that taurine may play an important role in suppressing effect by oxidized cholesterol and oxidized fish oil induced toxicity in rats.