碳氮源對浸漬培養金頂側耳胞外多醣之生成及碳水化合物組成之影響   全文下載

Effect of Carbon and Nitrogen Sources on the Production and Carbohydrate Composition of Exopolysaccharide by Submerged Culture of Pleurotus citrinopileatus

本研究探討不同碳、氮源對浸漬培養金頂側耳胞外多醣之生成影響及胞外多醣碳水化合物組成之變化。實驗結果顯示，生成金頂側耳菌絲體與胞外多醣的最佳碳、氮源為果糖與酵母蛋?白粉末。胞外多醣經水解後以氣相層析儀分析，結果顯示為異質多醣或多醣混合物，其碳水化合物組成為甘露糖、半乳糖、葡萄糖、木糖與岩藻糖，以甘露糖為主要組成。在不同培養條件下，金頂側耳生成的胞外多醣之碳水化合物組成百分比亦有所改變。

This study was aimed to investigate the effect of carbon and nitrogen sources on the production of exopolysaccharides (EPS) in submerged culture of Pleurotus citrinopileatus, and the variation of carbohydrate compositions in EPS during various culture conditions. The most suitable carbon and nitrogen sources for mycelial biomass and EPS production by P. citrinopileatus were fructose and yeast peptone powder. The carbohydrate compositions of EPS were determined to be mannose, galactose, glucose, xylose and fucose, which indicated EPS as heteroglycan or a mixture of glycans. The main carbohydrate composition in EPS from P. citrinopileatus was mannose after incubation of 14 days under 4% fructose and 2.5% yeast peptone powder. The carbohydrate composition of EPS from P. citrinopileatus changed with the supplementation of various carbon and nitrogen sources under submerged culture condition.