酒精在長期飲用中藥酒劑所引致肝臟損害之角色評估   全文下載

Role of Alcohol in the Induction of Hepatic Injury by Chronic Consumption of Medicated Wine

「金門益壽藥酒」為行政院衛生署中醫藥委員會公告的常用中藥酒劑之一。此藥酒係由17 種中藥材在含有25% 酒精溶液浸泡過濾，具有滋補強身的功效；在市場上廣被使用。本研究旨在瞭解25% 酒精所扮演的角色，因此，以胃管給藥，每天一次，連續12 週的方式，分別給予Wistar 大鼠2.08 mL/kg 濃度（在人體每日建議飲用的最低量）的「金門益壽藥酒」、不含酒精之藥酒組成中藥材溶液及25% 酒精溶液。由實驗結果發現，Wistar 大鼠連續飲用25% 酒精溶液（2.08 mL/kg/day）2 週後，血中與肝損傷或肝細胞壞死相關的指標數值，包括麩酸丙酮酸轉胺?（alanine transaminase）和麩酸草酸丙酮轉胺?（aspartate aminotransferase）以及肝臟中的羥基脯胺酸（hydroxyproline）濃度均有明顯上升的變化；同時，大鼠的肝臟出現嚴重纖維化現象。此外，連續飲用同量之「金門益壽藥酒」12 週後，與正常大鼠的數據相比，大鼠血中與肝損傷相關的指數雖有升高及呈現肝細胞傷害的現象，但是，其數值比單獨飲用25% 酒精溶液之大鼠為低。然而，單獨飲用不含酒精之藥酒組成中藥材溶液12 週後，大鼠並沒有出現任何肝功能損傷的情形。由此可知，即使在建議的飲用最低量，長期飲用25% 酒精是會造成肝臟的損傷。本實驗所得結果可提供相關單位訂定市售藥酒安全的飲用量及當為肝臟傷害防治的參考。

Kinmen Benefit Life medicated wine (KBLMW), a medicated wine with unified formulas proclaimed by the Committee on Chinese Medicine and Pharmacy (Department of Health, Executive Yuan, Taiwan), was made of 17 kinds of Chinese medicinal materials soaked in 25% alcohol. KBLMW has been widely used as conventional or complementary medicines for health regimen. In the present study, Wistar rats were administered once daily via gastric tube with KBLMW, de-alcoholic KBLMW (0% alcohol/ vol) or 25% alcohol at 2.08 mL/kg/day for 12 consecutive weeks, to clarify the role of alcohol in the induction of hepatic disorders by chronic consumption of KBLMW at the recommended intake amount. At the end of this treatment, the liver fibrosis in rats receiving 25% alcohol was found, featuring increase in serum levels of alanine transaminase and aspartate aminotransferase, as well as hepatic hydroxyproline contents. Comparing with the normal group, values of these serum indexes in KBLMW-fed rats elevated moderately but lower than those of 25% alcohol-fed rats. Also, the stage of liver fibrosis in KBLMW-treated rats was less advanced than that in 25% alcohol-treated group. In addition, the liver function parameters in rats receiving de-alcoholic KBLMW were close to those of normal group and liver fibrosis was not observed. The results suggest that alcohol is related to the induction of hepatic injury by chronic consumption of medicated wine even at the recommended daily intake amount. This finding can be used as the safety reference guide for clinical medicated wine drinking.