含聚果醣食品中膳食纖維含量分析方法之探討   全文下載

A Study on the Analytical Methods for Total Dietary Fiber in Fructan-Containing Foods

聚果醣包含菊糖與果寡糖，雖存在許多膳食纖維之生理功效，但卻因AOAC膳食纖維分析法之酵素重量法未能將其沉澱，故於食品標示時未能反映於膳食纖維數值上，因此本研究最主要之目的係對含聚果醣食品建立一套完善膳食纖維定量法，針對AOAC 985.29膳食纖維測定法與AOAC 999.03聚果醣測定法兩者合併之組合法作評估。藉由3種商業化聚果醣產品與實際添加聚果醣於蘆筍與茶飲料之測定結果確認本法之準確度，並進而計算精密度與量測不確定度。本方法之回收率可達97.02~101.35%，變異係數（CVs）介於0.63~1.17%。組合法之膳食纖維總量估算相較於僅用AOAC 985.29或AOAC 985.29加AOAC 999.03二者之結果顯著更接近實際值。

The fructans, inulin and oligofructose, were known to possess many of the physiologic properties of dietary fiber (DF). However, they were not listed as DF on labels of foods that contained them, because they cannot be completely recovered by the precipitate of AOAC enzymatic-gravimetric method. The main goal of this study was therefore to develop a quantitative method for total DF in fructan-containing foods. Evaluation was carefully done by combining AOAC 985.29 DF method and AOAC 999.03 fructans method. In addition to three commercial fructan products, asparagus and tea drink samples were selected and spiked with fructans to carry out the method validation. The results were all in excellent agreement with the expected values. Also, associated precision and measurement uncertainties were evaluated as well. Recoveries by the proposed method ranged from 97.02% to 101.35%, with coefficients of variation (CVs) of 0.63~1.17%. Total DF content was more accurate for combined method than either from AOAC 985.29 only or the sum of determining values from AOAC 985.29 plus AOAC 999.03.