利用固相萃取淨化及預濃縮方式前處理並以HPLC-UV、UV及原子吸收光譜檢測複合維他命錠劑中維生素B12   全文下載

Use of Solid Phase Extraction for Sample Clean-up and Preconcentration of Vitamin B12 in Multivitamin Tablet before HPLC-UV, UV and Atomic Absorption Spectrophotometry

本研究報導利用固相萃取淨化及預濃縮方式前處理複合維他命錠劑中維生素B12，後續以HPLC-UV、UV及原子吸收光譜進行檢測。固相參數中針對0.001 ppm維生素B12的最大負荷量及穿透體積分別為400 μg及1750 mL。HPLC的最適移動相為水/乙?（75:25，v/v，pH = 3.5＜以醋酸緩衝溶液調整＞，25°C）。HPLC-UV、UV及原子吸收光譜的檢測極限及線性範圍分別為0.1、0.1-25.0；0.4、0.4-5.0；2.7、2.7-300.0（μg/mL）。維生素B12的回收率約為96%。

A solid phase extraction method for sample clean-up and preconcentration of vitamin B12 in multivitamin tablet and its determination by HPLC-UV, UV and atomic absorption spectrophotometry is reported. Solid phase parameters such as maximum loading capacity and breakthrough volume were 400 μg and 1750 mL of 0.001 ppm for this vitamin respectively. A proper mobile phase used for HPLC was water/acetonitrile (75:25, v/v) with pH = 3.5 (adjusted with acetate buffer) at 25°C. Limit of detection and linear dynamic range for HPLC, UV and atomic absorption were 0.1, 0.1-25.0; 0.4, 0.4 -15.0; 2.7, 2.7-300.0 (μg/mL) respectively. Recovery of vitamin B12 was about 96%.