應用黨參炔苷和高效液相色譜指紋譜評價黨參質量   全文下載

The Use of Lobetyolin and HPLC-UV Fingerprints for Quality Assessment of Radix Codonopsis

黨參（Radix Codonopsis）是藥食兩用中藥材，有3個法定來源，即黨參Codonopsis pilosula、素花黨參C. pilosula var. modesta和川黨參C. tangshen的根。中國藥典2005年版將黨參炔?（lobetyolin）作為化學標示物用於黨參的TLC鑒定。本研究建立了一種將黨參炔?定量分析與HPLC-UV指紋圖譜定性分析相結合的方法，對桔梗科黨參屬8個種和其他屬4個種共44份樣品進行了分析，用於鑒定黨參、同屬代用品和同科偽品。結果顯示，除了黨參正品含有黨參炔?外，作為代用品的5個同屬植物中也含有該成分，甚至在同科其他3屬植物大花金錢豹（Campanumoea javanica）、桔梗（Platycodon grandiflorum）和半邊蓮（Lobelia chinensis）中也檢測到了黨參炔?，而前二者在市場上有時作為黨參摻偽品。含量分析顯示，黨參炔?在所分析樣品中的含量變化較大，在幾個代用品和摻偽品中也能檢測出較高的含量。據此結果，建議黨參炔?只作為黨參的一般化學標示物，而不能作為特徵化學標示物單獨用於黨參的鑒定。此外，依據紫外光譜的特徵選擇了包括黨參炔?在內的3類7個色譜峰組成了特徵峰，結果顯示3種黨參正品和5種同屬代用品的色譜圖中都含有這7個峰，而在同科其他屬4種樣品中有個別峰的缺失，因此可將黨參摻偽品與黨參正品及代用品區別開來，而黨參正品與代用品的區別方法還有待研究。

In 2005 edition of the Chinese Pharmacopoeia, lobetyolin was used as a marker for TLC identification of Radix Codonopsis, whose source plants are Codonopsis pilosula, C. pilosula var. modesta and C. tangshen. Quantification of lobetyolin in 44 samples derived from eight species of Codonopsis and another four genera of the same family Campanulaceae revealed that lobetyolin is present in all Codonopsis species and in Campanumoea javanica, Platycodon grandiflorum and Lobelia chinensis. In fact, the other five Codonopsis species are commonly used as the substitutes of Radix Codonopsis, and the roots of Campanumoea javanica and Platycodon grandiflorum are sometimes found as the adulterants. Contents of lobetyolin were found variable among different samples, and some substitutes and adulterants actually had comparably high contents of lobetyolin. Therefore, lobetyolin should be taken only as a general but not a definitive marker for the identification of Radix Codonopsis. HPLC-UV fingerprints with seven characteristic peaks were established for discriminating Radix Codonopsis from its adulterants, but more specific and definitive markers remain to be identified for differentiating Radix Codonopsis from its substitutes.