以簡易分光光度計法檢測製藥中Piroxicam及Propranolol   全文下載

A Simple Spectrofluorimetric Method for Determination of Piroxicam and Propranolol in Pharmaceutical Preparations

一項新穎、簡單、靈敏及快速的分光光度計法已被開發，用以檢測製藥中piroxicam及propranolol。本方法根據piroxicam及propranolol的氧化作用，使鈰由四價成為三價，其螢光偵測波長為352 nm，激發波長為255 nm。影響這些藥品氧化的變數亦被研究及最佳化。在本實驗條件下，piroxicam及propranolol的線性範圍分別為0.02-3.0及0.02-2.4 mg/L；檢測極限分別為0.006及0.008 mg/L；在濃度1.0 mg/L及五重覆之條件下，相對標準偏差分別為1.65及1.79%。添加回收率介於95-108%。此方法成功地應用在商業化製藥中的piroxicam及propranolol之檢測。

A new, simple, sensitive and rapid spectrofluorimetric method for the determination of piroxicam and propranolol in pharmaceutical formulations has been described. The method is based on the oxidation of piroxicam and propranolol with cerium (IV) to produce cerium (III), whose fluorescence was monitored at 352 nm while excited at 255 nm. The variables affecting oxidation of these drugs were studied and optimized. Under the experimental conditions, the calibration graphs were linear over the range of 0.02-3.0 and 0.02-2.4 mg/L, respectively, for piroxicam and propranolol. The limit of detection for piroxicam and propranolol was 0.006 and 0.008 mg/L, respectively, and the relative standard deviation of 5 replicate determinations of these drugs at 1.0 mg/L concentration level was 1.65 and 1.79%, respectively. Good recoveries in the range of 95-108% were obtained for spiked samples. The proposed methods were successfully applied to the determination of piroxicam and propranolol in commercial pharmaceutical formulations.