連續小波轉換應用於藥丸中 Oxfendazole 及 Oxyclozanide 混合物定量解析之重疊吸收訊號及比例訊號   全文下載

Continuous Wavelet Transform Applied to the Overlapping Absorption Signals and Their Ratio Signals for the Quantitative Resolution of Mixture of Oxfendazole and Oxyclozanide in Bolus

連續小波轉換（CWt）應用於藥丸中oxfendazole（oXF）及oxyclozanide（oXY）混合物定量解析之重疊吸收訊號及比例訊號，而不需使用任何化學前處理。針對訊號處理，墨西哥帽函數（mEXH）及morlet（morl）連續小波轉換在小波族群裡是合適的。首先，mEXH-CWt配合零點偵測技術直接應用於oXF及oXY藥物，在波長255-365 nm區間（oXY為328.7 nm，oXF為309.3 nm），藉由測量mEXH-CWt振幅得到此兩項藥物之校正圖。接著，利用morl-CWt分別找出oXY及oXF比例光譜之顯著波峰解析度。CWt振幅最大及最小值、波峰對波峰，以及零點偵測技術均使用於校正圖。藉由分析合成混合物及運用標準品添加技術，進行CWt-零點偵測及比例光譜-CWt技術確效，結果發現有良好的準確性、精確性及再現性。

Continuous wavelet transform (CWT) was applied to the overlapping absorption signals and their ratio signals for the quantitative resolution of mixture of oxfendazole (OXF) and oxyclozanide (OXY) in bolus without using any pre-chemical treatment. Mexican hat function (MEXH) and Morlet (MORL) continuous wavelet transforms (MEXH-CWT and MORL-CWT) for signal treatments were found to be suitable among the wavelet families. In the first step MEXH-CWT together with zero crossing technique is directly applied to the overlapping absorption spectra of OXF and OXY drugs and their calibration graphs were obtained by measuring the MEXH-CWT amplitudes at 328.7 nm for OXY and at 309.3 nm for OXF in the wavelet domain of 255-365 nm. In the second step MORL-CWT is used for finding the significant peak resolution of the ratio spectra of OXY and OXF respectively. The maxima and minima of CWT amplitudes and peak-to-peak and zero-crossing techniques were used for the calibration graphs. The method performances of CWT-zero crossing and ratio spectra-CWT techniques were validated by analyzing the synthetic mixtures and by applying the standard addition technique, and successfully accuracy, precision and reproducibility results were reported.