蘆薈萃取物（大黃素、蘆薈大黃素及蘆薈素）對調節活體小鼠腹膜巨噬細胞活性之探討   全文下載

Modulation of Activated Murine Peritoneal Macrophages Functions by Emodin, Aloe-emodin and Barbaloin Isolated from Aloe barbadensis

在活體小鼠腹膜巨噬細胞發炎系統中，一氧化氮合成酵素（inOs）是一個重要的指標物質。本研究發現從庫拉索蘆薈萃取出來三個重要成分：大黃素、蘆薈大黃素及蘆薈素，在老鼠巨噬細胞、脂多糖（lPs）及干擾素-γ（IFn-γ）所組成的發炎系統中，可以抑制系統所產生的一氧化氮（nO）、腫瘤壞死因子（tnF-α）和白血球間質素-12（Il-12）。結果顯示，大黃素、蘆薈大黃素及蘆薈素分別在濃度120，30 and 200 mM時，可以抑制一半濃度（IC50）的nO、tnF-α和Il-12。此外，也發現大黃素可以抑制發炎反應前期（pretreated lPs/IFn-γ）以及發炎反應後期（post-lPs/IFn-γ）nO的量。蘆薈大黃素以及蘆薈素可以抑制發炎反應前期nO的量。純化的化學物質鑑定由核磁共振儀（nMR）、質譜儀（mass spectral）以及沸點測定。

Activated murine macrophages produce high level of nitric oxide by inducible nitric oxide synthase (iNOS), which is an important mechanism in macrophage-induced inflammation. In the present study, we isolated emodin, aloe-emodin and barbaloin compounds from Aloe barbadensis and investigated their inhibitory effects on the production of nitric oxide (NO), tumor necrosis factor-α (TNF- α) and interleukin (IL-12) from murine macrophages activated by lipopolysaccharide (LPS) and interferon-gamma (IFN-γ). Our results showed that the 50% inhibition concentration (IC50) of emodin, aloe-emodin and barbaloin on NO, TNF-α and IL-12 from activated peritoneal macrophages were 120, 30 and 200 μM respectively. Our experimental results showed that reduction of NO level by emodin might inhibit both at the pretreated LPS/IFN-γ activation and post-LPS/IFN-γ activation pathway. Activities of aloe-emodin and barbaloin could be caused by inhibition at the pretreated LPS/IFN-γ activation event. Chemical structures of all the isolated components were identified by comparison of NMR, mass spectral data and melting points with those reported in literature.