利用高效液相層析儀分析樟芝Antrodia camphorata菌體之腺X、蟲草素與麥角固醇之含量   全文下載

Determination of Adenosine, Cordycepin and Ergosterol Contents in Cultivated Antrodia camphorata by HPLC Method

本研究是利用高效液相層析儀（HPLC）分析樟芝Antrodia camphorata菌體之腺?、蟲草素與麥角固醇之含量，分析方法包括藉由c18逆相層析管柱之梯度溶離及以甲醇為移動相之單一相溶離，層析時間可於20分鐘內完成。本試驗主要是針對樟芝BcRc 35396與BcRc 35398兩菌株，探討於5公升與100公升醱酵槽培養時，上述各成分之變化趨勢，同時亦比較700公升槽培養之醱酵液，經不同程序處理之乾菌體量，其腺?、蟲草素與麥角固醇含量之異同。

The concentrations of adenosine and cordycepin, 3’-deoxyadenosine in the hot water extract and ergosterol in the ethanol extract of a cultivated Antrodia camphorata were measured by high performance liquid chromatography (HPLC) method. The procedure were carried out on a reversed-phase C 18 column by linear gradient elution, using four eluents as mobile phase and by isocratic elution, using 100% methanol as mobile phase, respectively. The analysis was completed within 20 min. The application of this method for quantitation of adenosine and cordycepin in 5-L and 100-L fermentation from two A. camphorata strains BCRC 35396 and BCRC 35398 were described. C omparison of adenosine, cordycepin and ergosterol contents were made with different treatment of 700-L fermentation broth.