以高效液相層析及毛細管電泳分析葛根之組成成分   全文下載

Determination of Seven Pueraria Constituents by High Performance Liquid Chromatography and Capillary Electrophoresis

葛根為常見的中藥材，是豆科植物葛的乾燥根，含系列異黃酮類化合物，為主要的藥理活性成分。本研究開發高效液相層析（HPLC）及毛細管電泳（cE）方法，可分別在65及50 min內定量7個葛根組成成分。樣品使用5c18-MS管柱，流動相為磷酸二氫鉀（30 mM，以磷酸調至pH 3.0）及氰甲烷溶液之混合液，或使用毛細管（內徑75 μm）並以含10 mM磷酸二氫鈉與40 mM十二烷苯磺酸鈉之水溶液沖提。以重覆6次注射計算其積分面積比值之相對標準偏差，HPLC方法於同日（intraday）在0.50~0.97%之間，隔日（interday）在0.48~0.95%之間；cE方法則分別在0.88~1.25%（同日）及0.85~1.38%（隔日）之間。將兩分析方法應用於實際葛根樣品效果皆極良好，兩組結果之差距在5%以內。

Puerariae Radix is a commonly used C hinese herbal medicine derived from the dried root of the legume plant, which contains a series of isoflavones as its chief pharmacologically active constituents. U sing 7 pueraria constituents as marker substances, a high performance liquid chromatography (HPLC) and a capillary electrophoresis (CE) method were developed to assay the quality of Puerariae Radix within 65 and 50 min, respectively. Extracted samples were analyzed with a C osmosil 5C18-MS column and eluted with a mixture of potassium dihydrogenphosphate (30 mM, adjusted to pH 3.0 with phosphoric acid) and aqueous acetonitrile solution, or an uncoated capillary (75 μm I.D.) and carried with an aqueous solution containing 10 mM sodium dihydrogenphosphate and 40 mM sodium dodecyl sulphate. The relative standard deviation of the marker substances, on the basis of peak-area ratios for 6 replicate injections, was 0.50~0.97% (intraday) and 0.48~0.97% (interday) for HPLC, and 0.88~1.25% (intraday) and 0.85~1.38% (interday) for C E. Both methods yielded accurate results differing by only 5% when applied to the analysis of practical samples of Puerariae Radix.