玉米製品中伏馬毒素B1及B2之檢驗   全文下載

Determination of Fumonisin B1 and B2 in Corn Products

本研究以免疫親和性管柱萃取玉米及玉米製品中伏馬毒素B1及B2（以下簡稱FB1及FB2），續以高效液相層析儀進行定量分析，結果FB1、FB2之偵測極限分別為0.03 ppm及0.07 ppm；於玉米、生鮮玉米、玉米零食、玉米片中添加0.2-3.0 ppm之FB1，添加回收率為79.2-108.8%，相對標準偏差（RSD）為2.9-21.9%；添加0.2-3.0 ppm之FB2，其回收率為70.0-106.0%，RSD為4.7-20.0%。續於民國91年5-10月間，抽驗市售玉米（粒或粉）20件、生鮮玉米5件、玉米零食15件、玉米片10件、玉米澱粉5件、罐裝玉米5件及工廠用原料玉米16件，總共76件。結果顯示於20件玉米粒（或粉）中檢出7件含有伏馬毒素FB1及FB2污染量總和介於0.05-0.13 ppm；於5件生鮮玉米中檢出1件含有伏馬毒素0.15 ppm；於15件玉米零食中檢出2件，分別含有伏馬毒素0.5 ppm及0.16 ppm；於10件玉米片、5件玉米澱粉及5件罐裝玉米中皆未檢出；於16件工廠用原料玉米檢出1件含有伏馬毒素0.09 ppm。綜上所得，於76件檢體中檢出11件（14.5%）污染有FB1及/或FB2，污染量最高僅為0.16 ppm。

An analytical method using immunoaffinty column and HPLC was developed for determination fumonisins in corn and corn products. The detection limits for fumonisin B1 (FB1) and fumonisin B2 (FB2) were 0.03 ppm and 0.07 ppm, respectively. Spiked FB1 in dried corn (kernel and flour), fresh corn, corn snack and corn flake are at 0.2-3.0 ppm level, with recovery ranged from 79.2-108.8% and relative standard deviation (RSD) of 2.9-21.9%. FB2 spiked under the same condition as FB1 had recovery ranges of 70.0-106.0% and RSD of 4.7-20.0%. A total of 76 samples were collected, including 20 dried corn (kernel or flour), 5 fresh corn, 15 corn snacks, 10 corn flakes, 5 corn starch, 5 canned corn from commercial markets and 16 raw corn materials were purchased from corn snack manufacturers. From the total 76 samples, 11 samples (14.5%) were detected with FB1 and/or FB2. Seven samples of corn were at 0.05-0.13 ppm level, 1 fresh corn with 0.15 ppm, 2 corn snacks with 0.5 and 0.16 ppm, respectively, where as 1 corn raw material was at the 0.09 ppm level. The highest contamination was only 0.16 ppm. Nevertheless, no fumonisin was detected in corn flake, corn starch and canned corn samples.