以氣相層析質譜儀快速分析尿液中愷他命及其代謝物   全文下載

A Fast GC-MS Screening Procedure for Ketamine and Its Metabolites in Urine Samples

世界各地濫用愷他命的情況日益嚴重，急需一快速且精確的篩檢方法。目前仍無市售的免疫試劑可供篩檢之用。利用ketamine-d4為內標，經由液相-液相萃取且不衍生的方式，以氣相層析質譜儀進行分析。我們建立可同時分析愷他命及其代謝物：原愷他命及去氫原愷他命的篩檢方法。分析一個檢體的時間為5 min。愷他命、原愷他命及去氫原愷他命的偵測極限分別是10 ng/mL、10 ng/mL及30 ng/mL。分析低中高三種不同濃度（80, 160, 400ng/mL）同日內之精確度及準確度皆在-12.50%及8.17%以內。我們收集163個來自台灣迪斯可舞廳的尿液檢體。利用篩檢及確認分析方法分別檢驗此批檢體。利用確認分析方法分析一個檢體需20 min。此方法的靈敏度為98.9%，而特異性為100%。此單一步驟萃取的篩選方法提供了一個快速、靈敏且適用於法醫學及臨床毒理分析方法。"

The rapid increase of ketamine abuse worldwide has created a need for a fast and efficient screening procedure. Currently, there is no commercially available immunoassay. We developed a simple extraction and GC/MS screening procedure to screen out negative samples. Urine samples (1 mL) were spiked with ketamine-d4 as internal standard. After liquid-liquid extraction, samples were screened for ketamine (K) and its two metabolites, norketamine (NK) and dehydronorketamine (DHNK), with GC/MS without derivatization. The total analytical time is 5 min for each sample. The detection limit for K, NK and DHNK was 10 ng/mL, 10 ng/mL and 30 ng/mL, respectively. The within-run precision and accuracy for 80, 160, 400 ng/mL of K, NK and DHNK were all between -12.50% and 8.17%. We collected 163 urine samples from disco-dancing clubs in Taiwan. Samples were screened and confirmed with GC/MS. The total analytical time for GC-MS confirmation is 20 min per sample. The sensitivity is 98.9% and specificity is 100%. This screening method is rapid, sensitive and applicable to forensic and clinical toxicological analyses.