以cDNA微矩陣法偵測基因轉殖大豆種子及大豆加工產品   全文下載

Extending the cDNA Microarray Detection System to Evaluate Genetically Modified Soybean and Traditional Soy Foods

由於生物技術的快速發展，基因改良原料及食品已經越來越廣泛出現於各個國家的市面上。由於缺乏明確的標示，消費者基於社會、道德、及健康等方面的多重考量對於基因改良食品的排斥也越演越烈。各國政府為因應時勢的變化也提供了適當的法律規章以標示基因改良食品，給予消費者接受與否的選擇權。因此發展出一套快速而有效的基因改良食品檢驗工具及技術就顯得更為重要。這個實驗即是以cDNA微矩陣法檢測基因改良大豆原料及加工食品如豆漿、豆干和臭豆腐的研究，結果顯示，我們可以有效地偵測到一般常見於基因改良作物的外來DNA片段基因，如花椰菜鑲嵌病毒CaMV 35S啟動子，NOSt或35S終結子，nptII, hph或pat篩選基因，gus或gfp標示基因，CP4EPSPS等已知的轉殖基因和4個植物的內標準基因（如invertase, legumin, β-tubulin, actin基因）。此系統可以成功地區別轉殖大豆和未轉殖大豆，且對於基因組DNA已受損的加工食品如豆漿和豆干甚至發酵過的臭豆腐也能有效的偵測內含的外來基因。因此證明這個系統可以發展成一個快速便利的檢測技術。"

Aggravating controversies of GM (genetically modified) foods on the social, ethical and health aspects have lead to efficient and reliable detection systems to safeguard homogeneity of foods and address legal disputes. Improvising on the existing detection systems, the present investigation demonstrates the advantages of using cDNA microarray as a detection system for GM food. We have extended the study for the detection of genetic modification in commercial GM soybean seeds and in home-made traditional foods derived from these seeds, such as tofu and dried tofu. We looked for common T-DNA regions such as CaMV35S promoter, NOS or 35S terminators, nptII, hph or pat selection marker genes, GUS or GFP marker genes. We also searched for specific traits such as Bt11, T25, CP4EPSPS and four plant internal control genes (invertase, legumin, tubulin, actin gene). Results indicate that our microarray detection system can identify GM soybean seeds as well as processed food made from these seeds, with 100% accuracy. Transformation events identified in the GM soybean seeds were also visible in the processed foods, thereby confirming the accuracy and reproducibility of this procedure to even processed foods. We believe that with more popularity if the cDNA microarray detection system will soon be implemented as a diagnostic kit. 由於生物技術的快速發展，基因改良原料及食品已經越來越廣泛出現於各個國家的市面上。由於缺乏明確的標示，消費者基於社會、道德、及健康等方面的多重考量對於基因改良食品的排斥也越演越烈。各國政府為因應時勢的變化也提供了適當的法律規章以標示基因改良食品，給予消費者接受與否的選擇權。因此發展出一套快速而有效的基因改良食品檢驗工具及技術就顯得更為重要。這個實驗即是以cDNA微矩陣法檢測基因改良大豆原料及加工食品如豆漿、豆干和臭豆腐的研究，結果顯示，我們可以有效地偵測到一般常見於基因改良作物的外來DNA片段基因，如花椰菜鑲嵌病毒CaMV 35S啟動子，NOSt或35S終結子，nptII, hph或pat篩選基因，gus或gfp標示基因，CP4EPSPS等已知的轉殖基因和4個植物的內標準基因（如invertase, legumin, β-tubulin, actin基因）。此系統可以成功地區別轉殖大豆和未轉殖大豆，且對於基因組DNA已受損的加工食品如豆漿和豆干甚至發酵過的臭豆腐也能有效的偵測內含的外來基因。因此證明這個系統可以發展成一個快速便利的檢測技術。