以高效液相層析儀配合光電二極管陣列檢測器分析土耳其Isparta地區蘋果汁中棒麴黴素   全文下載

Determination of Patulin in Apple Juice Produced in Isparta, Turkey by HPLC with Diode Array Detection

本研究中，利用C18逆相層析管及高效液相層析儀於波長276 nm之紫外光下檢測棒麴黴素。棒麴黴素先經乙酸乙酯萃取，再以碳酸鈉溶液淨化。本試驗所使用的移動相及分離管柱可將棒麴黴素與干擾物質分離，特別是5-hydroxymethylfurfural。棒麴黴素於15至200 mg／L 濃度下所得到的標準曲線（ y = 327x - 292）之線性回歸常數為0.9998 （N = 6 ）。回收率經校正後顯示本研究為蘋果汁中棒麴黴素提供快速及方便之檢測方法。

In this study patulin was determined by HPLC on a reversed phase C18 column with UV detection at 276 nm. Patulin was extracted with ethyl acetate and then was cleaned up by extraction with sodium carbonate solution. The mobile phase and the column used in the HPLC determination were selected to separate patulin from the interfering substances in apple juice, especially 5-hydroxymethylfurfural (HMF). The linear regression coefficient of the standard curve (y = 327x – 292) for patulin at concentrations ranging from 15 to 200 μg/L was 0.9998 (N = 6). The results were corrected for the recovery percentage. The method is rapid and convenient for the determination of patulin in apple juice. 本研究中，利用C18逆相層析管及高效液相層析儀於波長276 nm之紫外光下檢測棒麴黴素。棒麴黴素先經乙酸乙酯萃取，再以碳酸鈉溶液淨化。本試驗所使用的移動相及分離管柱可將棒麴黴素與干擾物質分離，特別是5-hydroxymethylfurfural。棒麴黴素於15至200 mg／L 濃度下所得到的標準曲線（ y = 327x - 292）之線性回歸常數為0.9998 （N = 6 ）。回收率經校正後顯示本研究為蘋果汁中棒麴黴素提供快速及方便之檢測方法。