黃柏基原的化學辨識   全文下載

Chemical Identification on the Origins of Phellodendri Cortex

本研究由臺灣及大陸各地共收集28批市售黃柏樣品，先經顯微鏡檢確認其屬於關黃柏（Phellodendron amurense RUPRECHT）、川黃柏（P. chinense SCHNEID）、臺灣黃柏（P. wilsonii HAYATA et KANEHlRA） 及禿葉黃皮樹（P. chinense SCHNEID var. glabrius culum SCHNEID）等四品種藥材，然後利用毛細管電泳法分析所含五個主要生物鹼（berberine, palmatine, jatrorrhizine, phellodendrine及magnoflorine）成分含量，以建立黃柏基原的化學辨識方法。結果發現目前臺灣市面上的黃柏藥材多屬禿葉黃皮樹，此品種樣品含有相當高含量的生物鹼（18~38 mg／g），是最近幾年才引進市場的品項。本研究根據成分分析的結果，建立該品種的化學指紋鑑定圖譜，並利用前已歸納確立的化學辨識原則，驗證其他品種藥材。另外，本文綜合各品種黃柏的化學辨識方法，整理成一簡便的辨識流程圖。"

Twenty-eight samples of commercial articles of Phellodendri Cortex, originating from Phellodendron amurense RUPRECHT, P. chinense SCHNEID, P. wilsonii HAYATA et KANEHIRA, and P. chinense SCHNEID. var. glabrius culum SCHNEID, were collected from different herbal markets in Taiwan and Mainland China. The five alkaloids berberine, palmatine, jatrorrhizine, phellodendrine, and magnoflorine were determined by capillary electrophoresis for their contents in the samples, thereby a chemical identification method was established for identifying the sources of the drug materials. As this study turned out, P. chinense var. glabrius culum was found to be the predominant variety prevailing in Taiwan markets owing to their considerably high alkaloid contents (18~38 mg/g), and was currently introduced into Taiwan. With the analytical results obtained in this study, characteristic fingerprint for the variety of P. chinense var. glabrius culum was made and the suitability of the previously established chemical identification methods in the identification of other species of the drug materials were further verified. In addition, a facile identification flowchart based on the various chemical identification methods for Phellodendri Cortex was devised. 本研究由臺灣及大陸各地共收集28批市售黃柏樣品，先經顯微鏡檢確認其屬於關黃柏（Phellodendron amurense RUPRECHT）、川黃柏（P. chinense SCHNEID）、臺灣黃柏（P. wilsonii HAYATA et KANEHlRA） 及禿葉黃皮樹（P. chinense SCHNEID var. glabrius culum SCHNEID）等四品種藥材，然後利用毛細管電泳法分析所含五個主要生物鹼（berberine, palmatine, jatrorrhizine, phellodendrine及magnoflorine）成分含量，以建立黃柏基原的化學辨識方法。結果發現目前臺灣市面上的黃柏藥材多屬禿葉黃皮樹，此品種樣品含有相當高含量的生物鹼（18～38 mg／g），是最近幾年才引進市場的品項。本研究根據成分分析的結果，建立該品種的化學指紋鑑定圖譜，並利用前已歸納確立的化學辨識原則，驗證其他品種藥材。另外，本文綜合各品種黃柏的化學辨識方法，整理成一簡便的辨識流程圖。