利用微透析聯結高效能液相層析測定茶及茶品中之兒茶素   全文下載

The Application of On-line Microdialysis with High Performance Liquid Chromatography for Determining Catechins in Tea and Tea Drinks

本研究探討微透析聯結逆相高效能液相層析直接分析茶品中之兒茶素。茶樣品中之兒茶素擴散進入中空透析膜中之灌注液中，直接注射進入高效能液相層儀進行分析。C8層析管柱以乙?／pH4.0磷酸緩衝水溶液進行梯度沖提分離兒茶素，以UV diode-array偵測。研究探討影響透析效能之因素，包括灌注液與樣品溶液之條件，並且提出一個分析茶及茶品中之兒茶素成分的簡單快速方法。利用所提出之方法分析茶及茶品中之epicatechin、epicatechin gallate、epigallocatechin和epigallocatechin gallate。

A microdialysis on-line coupled to a reversed-phase liquid chromatography was investigated and applied for analyzing catechins in tea and tea drinks. Catechins in drink samples were diffused into the perfusate through a hollow fiber membrane and then on-line injected into the HPLC system. Catechins were separated with a C8 reversed column by gradient eluting using an acetonitrile/ phosphate buffer solution at pH 4.0, and monitored by a UV diode-array detector. Factors affecting the dialysis efficiency such as the conditions in perfusate and in the sample solution were evaluated. The proposed method provided a simple procedure for isolating catechins from tea and tea drink samples. The application was illustrated by the analysis of epicatechin, epicatechin gallate, epigallocatechin, and epigallocatechin gallate in tea drinks. 本研究探討微透析聯結逆相高效能液相層析直接分析茶品中之兒茶素。茶樣品中之兒茶素擴散進入中空透析膜中之灌注液中，直接注射進入高效能液相層儀進行分析。C8層析管柱以乙腈／pH4.0磷酸緩衝水溶液進行梯度沖提分離兒茶素，以UV diode-array偵測。研究探討影響透析效能之因素，包括灌注液與樣品溶液之條件，並且提出一個分析茶及茶品中之兒茶素成分的簡單快速方法。利用所提出之方法分析茶及茶品中之epicatechin、epicatechin gallate、epigallocatechin和epigallocatechin gallate。