農產品中依滅列殘留量檢驗方法之探討   全文下載

High Performance Liquid Chromatographic Determination of Imazalil Residue in Agricultural Products

本計畫利用醋酸乙酯萃取，經Bond Elut PRS及Bond Elut C18淨化處理後，以高效液相層析儀配合紫外光檢出器作為依滅列之檢驗方法，所使用之層析管柱為Inertsil ODS-3，以乙?：10 mM 磷酸二氫鉀溶液 （pH 2.5） = 35 : 65 做為移動相。其最低檢出限量為0.01 ppm。於油菜、高麗菜、新疆瓜、金桔、芒果及玉米分別添加0.05-3.0 ppm依滅列進行回收試驗，其回收率分別為油菜88.7~97.9%；高麗菜72.5~87.4%；新疆瓜81.4~90.5%；金桔81.9~87.2%；芒果75.0-85.3% ；玉米86.8~99.0%。將此方法應用於市售蔬果之殘留量分析，結果於20件檢體中均未檢出依滅列之殘留量。

A simple, rapid and reproducible residue analytical method for imazalil in fruits and vegetables has been developed. The method involved the use of an ion-exchange cartridge for sample clean up followed by high performance liquid chromatography with ultraviolet detector. Acetic ether was used for extraction. Bond Elut PRS and Bond Elut C18 were used for purification. The chromatography column used Inertsil ODS-3 and the mobile phase was created by acetonitrile-10 mM KH2PO3 solution (pH 2.5), in the ratio of 35:65. The detection limit is 0.01 ppm. Rape, cabbage, honeydew melon, cumquat, mango and corn were each spiked with 0.05-3.0 ppm imazalil to be compared with the recovery study. The yield was 88.7-97.9% for rape, 72.5-87.4% for cabbage, 81.4-90.5% for honeydew melon, 81.9-87.2% for cumquat, 75.0-85.3% for mango, and 86.8-99.0% for corn. When the method was applied in analyzing commercial fruits and vegetables, no imazalil residue was detected in 20 selected samples. 本計畫利用醋酸乙酯萃取，經Bond Elut PRS及Bond Elut C18淨化處理後，以高效液相層析儀配合紫外光檢出器作為依滅列之檢驗方法，所使用之層析管柱為Inertsil ODS-3，以乙腈：10 mM 磷酸二氫鉀溶液 （pH 2.5） = 35 : 65 做為移動相。其最低檢出限量為0.01 ppm。於油菜、高麗菜、新疆瓜、金桔、芒果及玉米分別添加0.05-3.0 ppm依滅列進行回收試驗，其回收率分別為油菜88.7～97.9%；高麗菜72.5～87.4%；新疆瓜81.4～90.5%；金桔81.9～87.2%；芒果75.0-85.3% ；玉米86.8～99.0%。將此方法應用於市售蔬果之殘留量分析，結果於20件檢體中均未檢出依滅列之殘留量。