台灣蜂膠乙醇萃出物之抗菌與清除DPPH自由基活性   全文下載

Antibacterial and DPPH Free Radical-scavenging Activities of the Ethanol Extract of Propolis Collected in Taiwan

本研究乃在探討台灣不同地區（台北、名間和枋寮）；不同時期（6, 8, 10-11月）所收集蜂膠乙醇萃出物之抗菌與抗氧化活性。此外並檢測這些活性之熱穩定性。結果發現，蜂膠乙醇萃出物清除DPPH自由基之抗氧化活性及對Staphylococcus aureus和Listeria monocytogenes抗菌活性，因產地及收穫期間之不同而有所差異。但對Escherichia coli及Salmonella typhimurium則未呈現抗菌效果。大致而言，以六月產台灣蜂膠之抗菌及抗氧化活性效果較佳。在所試之蜂膠樣品中則以名間六月產蜂膠之抗菌活性最強。而同時期台北產蜂膠所呈現之抗氧化活性最強。進一步以台北六月產蜂膠酒精萃出物進行試驗顯示，在50、80或100℃加熱一小時後，對DPPH自由基之清除能力顯著降低，但其抗菌活性則沒有顯著變化。"

In the present study, ethanolic extracts of propolis (EEP) collected from various regions (Taipei, Mingchen and Fangliao) in Taiwan during different time periods (June, August and October-November, 2000) were tested for their antibacterial and antioxidative activities. In addition, the thermal stabilities of these activities exerted by EEP were also investigated. It was found that the EEP samples, depending on collecting location and time period, exerted various antioxidative activities in terms of scavenging α,α-diphenyl-2-piorylhydrasyl (DPPH) free radicals and showed various extents of antibacterial activity against Staphylococcus aureus and Listeria monocytogenes, but not against Escherichia coli and Salmonella typhimurium. In general, the Taiwanese propolis extract collected in June showed the most profound antibacterial and free radical-scavenging activities than those collected during other time periods. Among all of the samples tested, EEP collected from the Mingchien area in June exhibited the highest antibacterial activity, while that collected from the Taipei area during the same time period showed the highest free radical-scavenging activity. Further tests of EEP collected from Taipei in June revealed that its DPPH free radical-scavenging effects reduced significantly after heating at 50, 80 or 100ûC for 1 hr, while its antibacterial activity remained unchanged. 本研究乃在探討台灣不同地區（台北、名間和枋寮）；不同時期（6, 8, 10-11月）所收集蜂膠乙醇萃出物之抗菌與抗氧化活性。此外並檢測這些活性之熱穩定性。結果發現，蜂膠乙醇萃出物清除DPPH自由基之抗氧化活性及對Staphylococcus aureus和Listeria monocytogenes抗菌活性，因產地及收穫期間之不同而有所差異。但對Escherichia coli及Salmonella typhimurium則未呈現抗菌效果。大致而言，以六月產台灣蜂膠之抗菌及抗氧化活性效果較佳。在所試之蜂膠樣品中則以名間六月產蜂膠之抗菌活性最強。而同時期台北產蜂膠所呈現之抗氧化活性最強。進一步以台北六月產蜂膠酒精萃出物進行試驗顯示，在50、80或100℃加熱一小時後，對DPPH自由基之清除能力顯著降低，但其抗菌活性則沒有顯著變化。