英文摘要 |
Ganoderma product has the highest sale volume in the health food market in Taiwan. The most widely-used raw material is the species of G. tsuage. Nine triterpenoids were isolated from the fruiting body of G. tsugae and their structures were identified, using 1H NMR and FT-IR, as ganoderic acid A (1), B (2), C (3), D (4), E (5), C5 (6), C6 (7), G (8) and ganoderenic acid D (9). A reverse-phase HPLC method was also established for the determination of these nine triterpenoids. Among these compounds, ganoderic acid E, C5, C6, G and ganoderenic acid D were analyzed for the first time by reverse-phase HPLC. The separation was carried out on a C18 reverse-phase column with a gradient elution of acetonitrile and 2% acetic acid (1/4 and 1/2, v/v) as mobile phase at a flow-rate of 0.8 mL/min and detection at 252 nm. Regression equation revealed the linear relationship (correlation coefficient: 0.9990~0.9999) between the peak-area ratios and concentrations of ganoderic acids. The recoveries of nine ganoderic acids were between 96.85~105.09%. The relative standard deviations of nine ganoderic acids range from 0.8~4.8% (intraday) and 0.7~5.1% (interday). The total contents of the nine ganoderic acids in eight Ganoderma samples were between 0.28~2.20%.
靈芝產品為國內保健食品之大宗,松杉靈芝(Ganoderma tsugae)子實體是最常使用的原料品種。本研究自松杉靈芝子實體中分離出九種三萜類(triterpenoids ),這些三萜類經氫核核磁共振化學位移,紅外線光譜圖及質譜鑑定後,確認為靈芝酸(ganoderic acid) A (1),B (2),C (3),D (4),E (5),C5 (6),C6 (7),G (8)及靈芝烯酸D (9) (ganoderenic acid D)。本研究並建立以逆相高效液相層析(reverse-phase HPLC)法,分析松杉靈芝原料中此九種三類的含量,其中靈芝酸E,C5,C6,G及靈芝烯酸D首次以HPLC分離定量。本實驗之高效液相層析法係採用C18逆相層析管柱,以梯度沖提方式分離,移動相為不同比例之乙腈:2%醋酸(1 : 2 及1 : 4,體積比),檢測波長為252 nm,流速為0.8 mL/min,得到良好分析結果。九種靈芝酸之線性回歸方程式之相關係數(r)皆大於0.9990 (n = 5),添加試驗回收率為96.85%~105.09%。同日內標準偏差為0.8~4.8%,異日間標準偏差為0.5~5.1%。以此九種三萜類總含量計算,利用上述液相層析法,檢測市售六種靈芝產品及二種松杉靈芝原料,其靈芝酸總含量為0.28%~2.2%。 |