| 英文摘要 |
For the evaluation of antioxidant activities of dipeptides (carnosine, anserine), and free amino acids (histidine, 1-methylhistidine, taurine, glycine, alanine, β-alanine), four methods were used, including the analysis of the inhibition of linoleic acid autoxidation, scavenging effect on α,α-diphenyl-β-picrylhydrazyl free radical, reducing power, and chelating ability of Cu2+. Results showed that carnosine and anserine were antioxidants preventing lipid peroxidation in linoleic acid systems. They also possessed effective abilities as free radical scavengers, reducing agents, and copper ion chelators. The activities increased with increasing concentration. However, the constituent amino acids of dipeptides, β-alanine, histidine and 1-methylhistidine, were not as effective in inhibiting oxidation regardless of individual or combined usage. Taurine, glycine, alanine and β-alanine showed much weaker antioxidant activities than carnosine, anserine, histidine and 1-methylhistidine. No synergistic effects on antioxidation were found among compounds used in combination. The results suggested that histidine-containing compounds were related to antioxidation abilities, and the peptide linkage between β-alanine, histidine and 1-methylhistidine was involved in the antioxidant activities of carnosine and anserine.
四種檢測方法包括抑制亞麻油酸自氧化、清除DPPH自由基、還原能力、螯合銅離子等被使用來評估雙胜肽（肌肽、甲肌肽）和游離胺基酸（組胺酸、1-甲基組胺酸、牛磺酸、甘胺酸、丙胺酸、b-丙胺酸）之抗氧化性，結果顯示肌肽及甲肌肽具有抑制亞麻油酸自氧化能力，且可作為良好之自由基清除劑、還原劑及銅離子整合劑，抗氧化活性隨濃度增加而增強，惟兩者之組成胺基酸包括b- 丙胺酸、組胺酸與1-甲基組胺酸之能力則不如雙胜肽。牛磺酸、甘胺酸、丙胺酸與b-丙胺酸之抗氧化能力遠低於肌肽、甲肌肽、組胺酸與1-甲基組胺酸，所有雙胜肽和游離胺基酸之混合皆無抗氧化之相乘效果。本研究顯示含組胺酸之相關化合物與抗氧化能力有關，而b-丙胺酸在與組胺酸、1-甲基組胺酸之胜肽鍵結合則與肌肽、甲肌肽之抗氧化活性有關。 |
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