溶劑萃取對口香糖中BHT及BHA氣相層析法定量之影響   全文下載

Effects of Extraction Solvent on Gas Chromatographic Quantitation of BHT and BHA in Chewing Gum

本研究採討不同溶劑萃取對口香糖中BHT及BHA氣相層析定量之影響。結果顯示在七種試驗溶劑中，乙醚能使口香糖膠塊呈現最佳之分散性，故對口香糖中之BHT及BHA具較佳之萃取效果。口香糖樣品（約1克）置於20 mL樣品瓶中加入5 mL乙醚及0.2 mL 0.5%之內標準品（ntetradecanol，14OH） 溶液，置於超音波振盪萃取一次5 min，即可達到最大之BHT及BHA萃取定量結果。添加100-200 μg之BHT及BHA於口香糖中，其回收率分別為99~101%（CV在1.5~3.2%）及94-99%（CV在7.1~8.6%）。每分析一個樣品僅需15-20 min，簡便又準確。以本方法分析市售15件口香糖中BHT及BHA之含量，結果顯示BHT含量為0~296 μg／g 及BHA含量為0~133 μg／g。口香糖在嘴裡咀嚼約15分鐘後，70%左右可溶性成分隨之溶出，而其中BHT及BHA之溶出量達約50%。

In this study, a simple and rapid method was developed for the determination of BHT (2,6-di-t-butyl-p-hydroxytoluene) and BHA (t-butyl-4-hydroxyanisole) in chewing gum using solvent extraction combined with gas chromatography. The results showed that among seven types of extraction solvent, diethyl ether was the best, as chewing gum dissolved and dispersed in diethyl ether, gave the highest yield of BHT and BHA from chewing gum. Recovery studies were performed on chewing gum, spiked with both BHT and BHA at 100~200µg/g each. The recoveries of both BHT and BHA were 99~101% (CV: 1.5~3.2%) and 94~99% (CV: 7.1~8.6), respectively. The coefficients of variation were less than 8.6%. Fifteen chewing gum samples were analyzed by the current method. Both the BHT and BHA contents were found to be 0~296 µg/g and 0~133 µg/g, respectively. After chewing for 15 min, 70% of the total soluble compounds and 50% of BHT and BHA were released from chewing gum. 本研究採討不同溶劑萃取對口香糖中BHT及BHA氣相層析定量之影響。結果顯示在七種試驗溶劑中，乙醚能使口香糖膠塊呈現最佳之分散性，故對口香糖中之BHT及BHA具較佳之萃取效果。口香糖樣品（約1克）置於20 mL樣品瓶中加入5 mL乙醚及0.2 mL 0.5%之內標準品（ntetradecanol，14OH） 溶液，置於超音波振盪萃取一次5 min，即可達到最大之BHT及BHA萃取定量結果。添加100-200 µg之BHT及BHA於口香糖中，其回收率分別為99～101%（CV在1.5～3.2%）及94-99%（CV在7.1～8.6%）。每分析一個樣品僅需15-20 min，簡便又準確。以本方法分析市售15件口香糖中BHT及BHA之含量，結果顯示BHT含量為0～296 µg／g 及BHA含量為0～133 µg／g。口香糖在嘴裡咀嚼約15分鐘後，70%左右可溶性成分隨之溶出，而其中BHT及BHA之溶出量達約50%。