利用毛細管電泳鑑定BCG疫苗   全文下載

Detection of BCG Vaccine by Capillary Electrophoresis

本研究利用核酸增幅法進行BCG疫苗基因體核酸之鑑定，使用毛細管電泳分析增幅之核酸片段，大約有350bp之PCR產物被確定。由於目前鑑定之方法大多十分費時與費力，使用CE分析PCR產物，除了方便、快速外，具有傳統電泳所缺乏可以核酸定量之優點。

We have used the polymerase chain reaction (PCR) to amplify specific DNA fragments of the Mycobacterium bovis BCG (BCG) vaccine from genomic DNA. We have used capillary electrophoresis (CE) to analyze and detect the PCR products. The ability to amplify a DNA fragment of about 350 bp will bring the speed and simplicity of PCR to vaccine identification in a genomic DNA, and even to quantitative DNA research. While current methods for determining the identity of BCG vaccine are very labor intensive, in this paper we will describe a PCR and CE technique for identifying the BCG vaccine. Thus, the identification of PCR products by CE may offer better resolving power, convenience, and quantitative capability than the conventional electrophoresis methods. 本研究利用核酸增幅法進行BCG疫苗基因體核酸之鑑定，使用毛細管電泳分析增幅之核酸片段，大約有350bp之PCR產物被確定。由於目前鑑定之方法大多十分費時與費力，使用CE分析PCR產物，除了方便、快速外，具有傳統電泳所缺乏可以核酸定量之優點。