以兩種互補的呈色法測定蜂膠中總類黃酮物質含量   全文下載

Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods

類黃酮具有多種生理活性，咸認是蜂膠中的關鍵成分。本研究利用氯化鋁反應及2,4-雙硝基苯?反應這兩種互補的呈色法，針對蜂膠中的類黃酮物質進行定量分析。結果顯示將兩種方法分別測得的含量相加後的總和可能較接近類黃酮物質的真正總含量。6件原膠中的類黃酮總含量在10.38 ± 0.14%至24.91 ± 0.53%之間，而就12件市售蜂膠產品所作的分析則發現，液態（酒精溶液）商品中的類黃酮總含量都在7%以下，粉末狀商品中的類黃酮總含量則在2.97 ± 0.05至22.73 ± 0.72%之間。"

Flavonoids, with various biological activities, are considered as key compounds in propolis. In this study, quantitative determinations of flavonoids in propolis were conducted by two complementary colorimetric methods, aluminum chloride method and 2,4-dinitrophenylhydrazine method. Results suggested that the sum of flavonoid contents determined by the above two individual methods may represent the real content of total flavonoids. In this work, six raw propolis samples were investigated and the total contents of flavonoids ranged from 10.38 ± 0.14% to 24.91 ± 0.53%. As for the 12 commercial propolis products examined, the levels of total flavonoids in tinctures were all below 7% and those in powdery products varied from 2.97 ± 0.05% to 22.73 ± 0.72%. 類黃酮具有多種生理活性，咸認是蜂膠中的關鍵成分。本研究利用氯化鋁反應及2,4-雙硝基苯肼反應這兩種互補的呈色法，針對蜂膠中的類黃酮物質進行定量分析。結果顯示將兩種方法分別測得的含量相加後的總和可能較接近類黃酮物質的真正總含量。6件原膠中的類黃酮總含量在10.38 ± 0.14%至24.91 ± 0.53%之間，而就12件市售蜂膠產品所作的分析則發現，液態（酒精溶液）商品中的類黃酮總含量都在7%以下，粉末狀商品中的類黃酮總含量則在2.97 ± 0.05至22.73 ± 0.72%之間。