高效液相層析分析嶺南野菊之黃酮類成分   全文下載

Analysis of Flavonoids in Vernonia Paltula by High-performance Liquid Chromatography

嶺南野菊（Vernonia Paltula）屬菊科植物，其乾燥全草是一種台灣的民間藥。本研究建立一個快速準確的高效液相層析分析方法，分析四種黃酮類成分（apigenin, apigenin-7-O-glucoside, luteolin =及luteolin-7-O-g1ucoside）。本實驗採用逆相層析管柱（Inertsil ODS-2），沖提液為乙?P0.1%（v／v）磷酸水溶液，檢測波長為254nm，內部標準品為ethylparaben作分析。四種黃酮類成分檢量線之相關係數（r）為0.9998至0.9999，均呈現良好線性關係。四種成分之回收率由89.3至95.6%，顯示準確性佳。本研究並探討四種黃酮類成分在嶺南野菊之花、葉、莖、根之含量，結果在葉部除apigenin-7-O-g1ucoside之含量低於花部外，其他三種成分之含量均最高；而根及莖部除含少量的apigenin-7-0-g1ucoside 外，均不合其他成分。嶺南野菊之四種成分之含量分別為apigenin-7-O-g1ucoside 0.6016 mg／g、apigenin 0.0042 mg／g、luteolin-7-O-g1ucoside 0.2160 mg／g 及luteolin 0.0577 mg／g。本研究同時採用高效液相層析方法，分析台灣另外三種Vernonia屬植物：一枝香（V. cinerea）、光耀藤（V. elliptica）及過山龍（V. gratiosa）。"

Lin-nan-yeh-chu, is the dried entire plant of Vernonia paltula (Compositae) and used as folk medicine in Taiwan. To evaluate the quality of V. paltula, a simple, rapid and accurate high-performance liquid chromatographic (HPLC) method was developed for the assay of four flavonoids apigenin (API), apigenin-7-O-glucoside (APG), luteolin (LUT) and luteolin-7-O-glucoside (LUG). The present HPLC system uses an Inertsil ODS-2 column by gradient elution with acetonitrile and 0.1% (v/v) phosphoric acid as the mobile phase. Ethyl paraben was used as an internal standard and detected at 254 nm. Regression equations revealed good linear relationships (correlation coefficients: 0.9998-0.9999) between the peak-area ratios of each constituent to ethyl paraben. The recovery of four marker constituents ranged from 89.3 to 95.6%. The contents of the four constituents in stem, flower, leaf and root parts of V. paltula have been compared. Leaf part consisted of the highest contents of flavonoids except for APG which is less than that in the flower. The root and stem only showed trace amount of APG but not the other three flavonoids. The contents of four constituents were 0.6016, 0.0042, 0.2160 and 0.0577 mg/g for apigenin-7-O-glucoside, apigenin, luteolin-7-O-glucoside and luteolin, respectively. HPLC chromatograms of another three plants of genus of Vernonia in Taiwan, V. cinerea, V. elliptica and V. gratiosa, have also been compared. 嶺南野菊（Vernonia Paltula）屬菊科植物，其乾燥全草是一種台灣的民間藥。本研究建立一個快速準確的高效液相層析分析方法，分析四種黃酮類成分（apigenin, apigenin-7-O-glucoside, luteolin =及luteolin-7-O-g1ucoside）。本實驗採用逆相層析管柱（Inertsil ODS-2），沖提液為乙腈P0.1%（v／v）磷酸水溶液，檢測波長為254nm，內部標準品為ethylparaben作分析。四種黃酮類成分檢量線之相關係數（r）為0.9998至0.9999，均呈現良好線性關係。四種成分之回收率由89.3至95.6%，顯示準確性佳。本研究並探討四種黃酮類成分在嶺南野菊之花、葉、莖、根之含量，結果在葉部除apigenin-7-O-g1ucoside之含量低於花部外，其他三種成分之含量均最高；而根及莖部除含少量的apigenin-7-0-g1ucoside 外，均不合其他成分。嶺南野菊之四種成分之含量分別為apigenin-7-O-g1ucoside 0.6016 mg／g、apigenin 0.0042 mg／g、luteolin-7-O-g1ucoside 0.2160 mg／g 及luteolin 0.0577 mg／g。本研究同時採用高效液相層析方法，分析台灣另外三種Vernonia屬植物：一枝香（V. cinerea）、光耀藤（V. elliptica）及過山龍（V. gratiosa）。