市售清涼飲料中黃樟素之簡易快速氣相層析定量分析法   全文下載

A Rapid and Simple Gas Chromatographic Method for Direct Determination of Safrole in Soft Drinks

本研究建立了市售清涼飲料中黃樟素之簡便快速氣相層析方法。選擇水溶性之1,4-dihydroxybenzene（DHB）為內標準加入清涼飲料中，樣品不需經任何前處理，直接注入氣相層析儀中，採用直接注入（direct injection）之方式，配合中間極性之CP-SIL 8CB管柱（30 m × 0.53 mm， 1.5 μm）分析定量清涼飲料之黃樟素。最低檢出量為0.25 μg／mL左右。添加safrole 及isosafrole 5.0 及10.0 μg／mL於1 mL檢體中，直接注入GC分析，其回收率為98~108%，變異係數cv%在8.7% 以下。以本方法分析市售不同廠牌之清涼飲料中黃樟素含量。結果顯示25 件清涼飲料中有20 件檢測出含safroleh 及／或isosafrole 。且safrole 之含量均超過食品添加物使用範圍及用量標準之規定（1 μg／mL）3-5 倍。"

A simple and rapid method was developed to determine the safrole and isosafrole in soft drink using a megapore semi-polar column (CP-SIL 8CB, 30 m × 0.53 mm, 1.5 μm) with direct injection gas chromatography. Direct quantitative analysis of safrole and isosafrole in soft drinks was carried out without any sample pretreatment procedure. The water soluble compound 1,4-dihydroxybenzene (DHB) was used as the internal standard. The detection limit for safrole and isosafrole was 0.25 μg/mL. A recovery study was performed using one of the soft drinks by spiking 1 mL with safrole and isosafrole at 5.0 and 10.0 μg, respectively. The recovery was found in the range of 98-108% with coefficients of variation being less than 8.7%. Twenty-five commercial soft drinks were analyzed by the current method, and results indicated that 20 out of 25 soft drink samples contained safrole andlor isosafrole, and the amount of safrole was 3-5 fold over the regulated amount, 1 μg/mL. 本研究建立了市售清涼飲料中黃樟素之簡便快速氣相層析方法。選擇水溶性之1,4-dihydroxybenzene（DHB）為內標準加入清涼飲料中，樣品不需經任何前處理，直接注入氣相層析儀中，採用直接注入（direct injection）之方式，配合中間極性之CP-SIL 8CB管柱（30 m × 0.53 mm， 1.5 μm）分析定量清涼飲料之黃樟素。最低檢出量為0.25 μg／mL左右。添加safrole 及isosafrole 5.0 及10.0 μg／mL於1 mL檢體中，直接注入GC分析，其回收率為98～108%，變異係數cv%在8.7% 以下。以本方法分析市售不同廠牌之清涼飲料中黃樟素含量。結果顯示25 件清涼飲料中有20 件檢測出含safroleh 及／或isosafrole 。且safrole 之含量均超過食品添加物使用範圍及用量標準之規定（1 μg／mL）3-5 倍。